Onarheim H, Brofeldt B T, Gunther R A
Department of Physiology, University of Bergen, Norway.
Burns. 1996 May;22(3):212-6. doi: 10.1016/0305-4179(95)00127-1.
Hyaluronam (HYA) (formerly hyaluronic acid) is an important constituent of the interstitial matrix in skin. Following major burn injury plasma HYA is known to increase markedly. The present study investigated to what extent the lymphatic removal of HYA from skin is affected following major burn injuries. Under ketamine anaesthesia a 30 per cent full thickness scald injury was inflicted on sheep previously provided with a chronic prefemoral lymph fistula. Animals were resuscitated for 24 h using lactated Ringer's infusion as needed to restore and maintain cardiac output within +/- 10 per cent of baseline. Following burn injury, lymph flow from thermally injured tissue gradually increased to 10-25 times above baseline. The baseline HYA concentration in lymph was 4.3 +/- 1.1 micrograms/ml; the HYA concentration in lymph was not significantly altered postinjury. The lymphatic HYA flux (flow x concentration) increased postinjury to 5-30 times control. The HYA concentration in plasma was 162 +/- 12 ng/ml at baseline; postinjury plasma HYA peaked at two to three times baseline at 4-8 h after the injury. At 24 h postinjury plasma HYA was reduced compared to baseline. In conclusion, after major burn injury the lymphatic transport of HYA from the injured area into the systemic circulation increased markedly, leading to elevated plasma concentrations of this high molecular weight connective tissue component.
透明质酸（HYA）（以前称为透明质酸）是皮肤间质基质的重要组成部分。已知在严重烧伤后血浆中的透明质酸会显著增加。本研究调查了严重烧伤后皮肤中透明质酸的淋巴清除受到何种程度的影响。在氯胺酮麻醉下，对先前患有慢性股前淋巴瘘的绵羊造成30%的全层烫伤。根据需要使用乳酸林格氏液输注对动物进行24小时复苏，以将心输出量恢复并维持在基线的±10%以内。烧伤后，来自热损伤组织的淋巴流量逐渐增加至基线以上的10 - 25倍。淋巴中透明质酸的基线浓度为4.3±1.1微克/毫升；损伤后淋巴中透明质酸的浓度没有显著变化。淋巴中透明质酸的通量（流量×浓度）在损伤后增加至对照的5 - 30倍。血浆中透明质酸的基线浓度为162±12纳克/毫升；损伤后血浆透明质酸在损伤后4 - 8小时达到基线的两到三倍峰值。损伤后24小时，血浆透明质酸与基线相比有所降低。总之，严重烧伤后，透明质酸从损伤区域向体循环的淋巴转运显著增加，导致这种高分子量结缔组织成分的血浆浓度升高。
