Graham J K
Department of Physiology, Colorado State University, Fort Collins, USA.
Vet Clin North Am Equine Pract. 1996 Apr;12(1):111-7. doi: 10.1016/s0749-0739(17)30298-5.
Methodologies to capacitate bovine spermatozoa, induce the acrosome reaction, and fertilize bovine oocytes in vitro have been established. The capability to do the same with stallion spermatozoa, however, is not available. Several different methods have been used to capacitate stallion spermatozoa with variable results. More basic research needs to be done to establish in vitro conditions necessary to capacitate and induce an acrosome reaction in stallion spermatozoa. Although much progress can be expected in this area, it is unlikely that the general practitioner will use these technologies in the near future.
已建立了使牛精子获能、诱导顶体反应以及在体外使牛卵母细胞受精的方法。然而,对马精子进行同样操作的能力尚不存在。已使用几种不同方法使马精子获能,但结果各异。需要开展更多基础研究,以确定使马精子获能并诱导顶体反应所需的体外条件。尽管在这一领域有望取得很大进展,但普通从业者近期不太可能使用这些技术。