Akeo K, Hiramitsu T, Kanda T, Karasawa Y, Okisaka S
Department of Ophthalmology, National Defense Medical College, Saitama, Japan.
Ophthalmic Res. 1996;28(1):8-18. doi: 10.1159/000267868.
The rod outer segments of the retina that are phagocytized by retinal pigment epithelial (RPE) cells are susceptible to lipid peroxidation because of their high content of polyunsaturated fatty acids. Linoleic hydroperoxides (LHP), synthesized by peroxidation of linoleic acids (LA), produce greater damage to retinal function than does LA. We compared the effects of LHP and LA on the growth of cultured chick embryonic RPE cells and analyzed a model of data sets using multiple linear regression for the number of cells as a function of time. The spectrum of LA had a sharp peak at 205 nm and a broad spectrum at 235 nm, while LHP had only a broad spectrum at 235 nm. Exposure to LA and LHP caused dose-dependent damage of chick embryonic RPE cells: they were significantly more affected by the addition of LHP than LA. The antioxidative enzymes catalase and superoxide dismutase minimized damage to the RPE cells caused by LHP in proportion to the enzyme concentration. However, RPE cells incubated with LA were more affected by the enzymes than when no enzymes were added.
视网膜色素上皮（RPE）细胞吞噬的视网膜视杆细胞外段，由于其富含多不饱和脂肪酸，易发生脂质过氧化。由亚油酸（LA）过氧化合成的氢过氧化亚油酸（LHP），对视网膜功能的损害比LA更大。我们比较了LHP和LA对培养的鸡胚RPE细胞生长的影响，并使用多元线性回归分析了数据集模型，将细胞数量作为时间的函数。LA的光谱在205nm处有一个尖峰，在235nm处有一个宽谱，而LHP在235nm处只有一个宽谱。暴露于LA和LHP会导致鸡胚RPE细胞的剂量依赖性损伤：与LA相比，添加LHP对它们的影响明显更大。抗氧化酶过氧化氢酶和超氧化物歧化酶按酶浓度比例将LHP对RPE细胞的损伤降至最低。然而，与未添加酶时相比，用LA孵育的RPE细胞受酶的影响更大。
