Akeo K, Nagasaki K, Tanaka Y, Curran S A, Dorey C K
Department of Ophthalmology, School of Medicine, Keio University, Tokyo, Japan.
Ophthalmic Res. 1992;24(6):357-64. doi: 10.1159/000267194.
We assayed the proliferation of porcine retinal pigment epithelial (RPE) cells, bovine melanotic and amelanotic RPE cells, and bovine aortic endothelial cells exposed to 20, 10 and 5% oxygen and compared their responses to oxygen and antioxidative enzymes (superoxide dismutase and catalase). Irrespective of the cell type, the cell growth was optimal in 10% oxygen that is most closely approximating to the oxygen concentration prevailing in the cellular environment of the choroid and the retina in vivo. However, the effects of oxygen concentrations were cell specific because bovine endothelial cells were influenced by lowering of oxygen concentrations more significantly than bovine and porcine RPE cells. Moreover, addition of antioxidative enzymes caused significant improvement in growth of porcine RPE cells, but had no significant effects on bovine RPE cells. On the contrary, the bovine vascular endothelial cells represented the only one cell type significantly inhibited by antioxidative enzymes, i.e., a decrease in reactive intermediates of oxygen was seen in the media. Our results show that responses of vascular endothelial cells to reactive species of oxygen were distinctly different from those of RPE cells and more easily influenced by the environment related to hypoxia than RPE cells.
我们检测了暴露于20%、10%和5%氧气环境下的猪视网膜色素上皮（RPE）细胞、牛黑色素性和无黑色素性RPE细胞以及牛主动脉内皮细胞的增殖情况，并比较了它们对氧气和抗氧化酶（超氧化物歧化酶和过氧化氢酶）的反应。无论细胞类型如何，在10%氧气环境下细胞生长最佳，该氧气浓度最接近体内脉络膜和视网膜细胞环境中普遍存在的氧气浓度。然而，氧气浓度的影响具有细胞特异性，因为牛内皮细胞比牛和猪RPE细胞更显著地受到氧气浓度降低的影响。此外，添加抗氧化酶可使猪RPE细胞的生长显著改善，但对牛RPE细胞没有显著影响。相反，牛血管内皮细胞是唯一一种受到抗氧化酶显著抑制的细胞类型，即培养基中可见氧反应中间体减少。我们的结果表明，血管内皮细胞对活性氧的反应与RPE细胞明显不同，并且比RPE细胞更容易受到与缺氧相关环境的影响。
