Ichikawa S, Shiozawa Y, Uchino S
Department of Anatomy, Tokyo Medical College, Japan.
Arch Histol Cytol. 1996 Mar;59(1):87-96. doi: 10.1679/aohc.59.87.
Perivascular accumulation of leukocytes in the lung was induced by intratracheal administration of sheep erythrocyte antigen to primed mice. The route of migration of intravascular leukocytes to the perivascular space in the lung, in particular from arteries, and the structure of lymphatic vessels among the aggregated leukocytes were examined by transmission electron microscopy. Leukocytes--lymphocytes, granulocytes, monocytes and macrophages--were demonstrated to adhere to the endothelial surface and to migrate between endothelial cells to reach the internal elastic lamina of arteries. Becoming conspicuously constricted, the leukocytes penetrate through this elastic lamina. They further migrate through the smooth muscle layer to the interstitium, passing through the external elastic laminar region. At 2 days after antigen administration, dilated lymphatic vessels containing large numbers of leukocytes in the lumen and bearing endothelial gaps open to the interstitium began to be seen. The lymphatic walls were more convoluted and richer in pinocytotic vesicles than those prior to antigen challenge. This study confirms the light microscopic findings by Curtis et al. (1990) that arteries, besides veins, venules and capillaries, may represent a major route of inflammatory cell entry into the lung parenchyme in an acute and vigorous immune response. In addition, lymphatic vessels were suggested to be newly formed for the transport of fluid and immune cells from the sites of inflammation in the lung.
通过向致敏小鼠气管内注射绵羊红细胞抗原，诱导肺部血管周围白细胞积聚。通过透射电子显微镜检查血管内白细胞迁移至肺部血管周围间隙的途径，特别是从动脉迁移的途径，以及聚集白细胞之间淋巴管的结构。结果表明，白细胞（淋巴细胞、粒细胞、单核细胞和巨噬细胞）粘附在内皮表面，并在内皮细胞之间迁移，到达动脉的内弹性膜。白细胞明显收缩，穿透该弹性膜。它们进一步穿过平滑肌层到达间质，穿过外弹性膜区域。抗原注射后2天，开始出现扩张的淋巴管，管腔内含有大量白细胞，其内皮间隙通向间质。与抗原攻击前相比，淋巴管壁更加曲折，吞饮小泡更丰富。本研究证实了Curtis等人（1990年）的光学显微镜观察结果，即在急性强烈免疫反应中，除静脉、小静脉和毛细血管外，动脉可能是炎症细胞进入肺实质的主要途径。此外，研究表明淋巴管是新形成的，用于从肺部炎症部位运输液体和免疫细胞。
