一种用于甘油脂质脂肪酸快速分析的改进方法。
An improved method for rapid analysis of the fatty acids of glycerolipids.
作者信息
Ichihara K, Shibahara A, Yamamoto K, Nakayama T
机构信息
Department of Agricultural Chemistry, Kyoto Prefectural University, Japan.
出版信息
Lipids. 1996 May;31(5):535-9. doi: 10.1007/BF02522648.
An improved rapid procedure to determine the fatty acid composition of glycerolipids is described. The procedure includes KOH-catalyzed transesterification and high-speed gas chromatography. Glycerolipids (20-40 mg) were mixed with 2 mL of hexane and 0.2 mL of 2 M methanolic KOH at room temperature for 1-2 min. The fatty acid methyl esters in the hexane layer were analyzed by gas chromatography on 10% SP-2340 at 240 degrees C. Methyl linolenate and docosahexaenoate eluted within 2 and 5 min, respectively. Analysis was thus completed within 5 min for common vegetable oils and 8 min for fish oils.
描述了一种改进的快速测定甘油脂脂肪酸组成的方法。该方法包括氢氧化钾催化的酯交换反应和高速气相色谱法。将甘油脂（20 - 40毫克）与2毫升己烷和0.2毫升2M的甲醇氢氧化钾在室温下混合1 - 2分钟。己烷层中的脂肪酸甲酯通过在240℃下使用10% SP - 2340的气相色谱法进行分析。亚麻酸甲酯和二十二碳六烯酸甲酯分别在2分钟和5分钟内洗脱。因此，对于常见植物油，分析在5分钟内完成，对于鱼油则在8分钟内完成。
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