Lewis M P, Clements M, Takeda S, Kirby P L, Seki H, Lonsdale L B, Sullivan M H, Elder M G, White J O
Institute of Obstetrics & Gynaecology, Royal Postgraduate Medical School, Hammersmith Hospital, London, UK.
Placenta. 1996 Mar-Apr;17(2-3):137-46. doi: 10.1016/s0143-4004(96)80006-3.
Many previous studies in both mouse and human placenta have implicated a role for colony stimulating factor-1 (CSF-1) in the regulation of placental development. In this study we have examined CSF-1 production by an immortalized cell line (TCL-1) derived from the choriodecidua, transfected with a retrovirus gene coding for the large-T antigen. TCL-1 cells were uniformly positive by immunocytochemistry for the composite sub-units of human chorionic g gonadotrophin (hCG) but were negative for markers of other cell types localized at the fetal-maternal interface. Gelatinase enzymes were secreted by TCL-1 cells cultured on extracellular matrix in a manner indicative of extra-villous trophoblast. Dot-blot immunoassays and ELISA indicated that CSF-1 was secreted by TCL-1 cells, at levels comparable to primary trophoblast cells and BeWo choriocarcinoma (trophoblast tumour) cells. Reverse transcriptase-polymerase chain reaction analysis confirmed the presence in TCL-1 cells of CSF-1 receptor mRNA (c-fms gene product), indicating that the components of a potential autocrine loop were present in these cells. Proliferation of TCL-1 cells was not affected by the addition of exogenous CSF-1 but was elevated in response to treatment with a CSF-1 neutralizing antibody. The immortalized cell line, TCL-1, provides a potential model in which to investigate regulation of growth and differentiation of trophoblast cells in vitro.
此前许多针对小鼠和人类胎盘的研究表明，集落刺激因子-1（CSF-1）在胎盘发育调控中发挥作用。在本研究中，我们检测了源自绒毛蜕膜、经编码大T抗原的逆转录病毒基因转染的永生化细胞系（TCL-1）产生CSF-1的情况。免疫细胞化学检测显示，TCL-1细胞对人绒毛膜促性腺激素（hCG）的复合亚基呈均匀阳性，但对位于母胎界面的其他细胞类型标志物呈阴性。TCL-1细胞在细胞外基质上培养时分泌明胶酶，其方式表明为绒毛外滋养层细胞。斑点印迹免疫分析和酶联免疫吸附测定表明，TCL-1细胞分泌CSF-1，其水平与原代滋养层细胞和BeWo绒毛膜癌（滋养层肿瘤）细胞相当。逆转录聚合酶链反应分析证实TCL-1细胞中存在CSF-1受体mRNA（c-fms基因产物），表明这些细胞中存在潜在自分泌环的成分。添加外源性CSF-1对TCL-1细胞的增殖没有影响，但用CSF-1中和抗体处理后细胞增殖增加。永生化细胞系TCL-1为体外研究滋养层细胞生长和分化的调控提供了一个潜在模型。
