Huangfu Y M, Zhang D J, Cheng J Z, Qian M, Liang J Q, Li D
Department of Medical Molecular Biology, Tongji Medical University, Wuhan.
J Tongji Med Univ. 1995;15(3):138-42. doi: 10.1007/BF02888221.
By employing the pUC19 as a backbone, the regulatory and signal sequences which encode kanamycin resistance, and mycobacterial plasmid origin of replication (oriM) were cloned into the pUC19. The recombinant E. Coli-mycobacteria shuttle expression plasmid pBCG-8000 was constructed. The pBCG-8000 was able to replicate in both E. Coli and mycobacteria (including BCG) systems, and to confer stable kanamycin resistance upon transformants. The study should facilitate the development of BCG and other mycobacteria into multivalent vaccine vectors.
以pUC19为骨架，将编码卡那霉素抗性的调控和信号序列以及分枝杆菌质粒复制起点（oriM）克隆到pUC19中。构建了重组大肠杆菌-分枝杆菌穿梭表达质粒pBCG-8000。pBCG-8000能够在大肠杆菌和分枝杆菌（包括卡介苗）系统中复制，并赋予转化体稳定的卡那霉素抗性。该研究应有助于将卡介苗和其他分枝杆菌开发成多价疫苗载体。
