在零下温度下形成稳定的结晶酶-底物中间体。
Formation of stable crystalline enzyme-substrate intermediates at sub-zero temperatures.
作者信息
Fink A L, Ahmed A I
出版信息
Nature. 1976 Sep 23;263(5575):294-7. doi: 10.1038/263294a0.
DOI:10.1038/263294a0
PMID:8732
Abstract
Sub-zero temperatures can be used to trap intermediates in enzyme-catalysed reactions using suitable cryosolvents. The feasibility of obtaining such intermediates in the crystalline state for X-ray diffraction studies has been demonstrated with several proteases, using specific substrates and optimal pH.
摘要
利用合适的低温溶剂，可通过零下温度捕获酶催化反应中的中间体。使用特定底物和最佳pH值，已通过几种蛋白酶证明了获得用于X射线衍射研究的晶体状态中间体的可行性。
Zotero 插件