弹性蛋白酶-底物复合物在零下55摄氏度时的晶体结构。
Crystal structure of elastase-substrate complex at -- 55 degrees C.
作者信息
Alber T, Petsko G A, Tsernoglou D
出版信息
Nature. 1976 Sep 23;263(5575):297-300. doi: 10.1038/263297a0.
DOI:10.1038/263297a0
PMID:958484
Abstract
The structure of a specific acyl-enzyme intermediate in the elastase-catalysed hydrolysis of N-carbobenzoxy-L-alanyl-p-nitrophenol ester has been determined by X-ray diffraction at 3.5 A resolution. The acyl-enzyme was stabilised by cooling the crystal to --55 degrees C during substrate addition and data collection.
摘要
通过3.5埃分辨率的X射线衍射确定了弹性蛋白酶催化N-苄氧羰基-L-丙氨酰对硝基苯酚酯水解过程中特定酰基酶中间体的结构。在添加底物和收集数据期间，通过将晶体冷却至-55℃来稳定酰基酶。
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