Da J, Chen L, Hu Y
Department of Pathology, Air Force General Hospital, Beijing.
Zhonghua Zhong Liu Za Zhi. 1996 Jan;18(1):27-9.
Surgical samples from 40 cases of primary lung cancer were studied by in situ hybridization and polymerase chain reaction (PCR) to detect HPV-DNA in different types of lung cancer. In addition, PCR-RFLP was used to examine mutation of p53 exon 7. The results showed that HPV-DNA positive rate in lung cancer was 55% (22/40 cases), including SCLC (9/9 cases), squamous cell carcinomas (8/16 cases), and adenocarcinomas (5/12 cases). Amplification of p53 exon 7 was seen in 5 of 22 HPV-DNA positive cases. RFLP analysis showed that p53 exon 7 mutation was present in two cases with gene amplification. In HPV-DNA negatives there was only one case with exon 7 amplification but no mutation. SCLC and squamous carcinoma had higher HPV infection rate than other types of lung cancer. The results suggest some relation between HPV infection and p53 gene mutation.
采用原位杂交和聚合酶链反应（PCR）技术对40例原发性肺癌手术标本进行研究，以检测不同类型肺癌中的人乳头瘤病毒（HPV）-DNA。此外，用PCR-限制性片段长度多态性（RFLP）法检测p53基因第7外显子的突变情况。结果显示，肺癌中HPV-DNA阳性率为55%（22/40例），其中小细胞肺癌（SCLC）为9/9例，鳞状细胞癌为8/16例，腺癌为5/12例。22例HPV-DNA阳性病例中有5例出现p53基因第7外显子扩增。RFLP分析显示，2例基因扩增病例存在p53基因第7外显子突变。在HPV-DNA阴性病例中，仅1例出现第7外显子扩增，但无突变。SCLC和鳞状细胞癌的HPV感染率高于其他类型肺癌。结果提示HPV感染与p53基因突变之间存在一定关系。
