应用聚合酶链反应检测支气管源性癌中人乳头瘤病毒16、18型DNA相关序列

Detection of human papillomavirus types 16, 18 DNA related sequences in bronchogenic carcinoma by polymerase chain reaction.

作者信息

Li Q, Hu K, Pan X, Cao Z, Yang J, Hu S

机构信息

Department of Respiration, First Affiliated Hospital of Hubei Medical University, Wuhan.

出版信息

Chin Med J (Engl). 1995 Aug;108(8):610-4.

PMID:7587494

Abstract

In studying the relationship between human papillomavirus (HPV) and bronchogenic carcinoma, "high-risk" HPV 16, 18 DNA sequences were detected in samples from 50 lung cancer patients, 18 patients with benign pulmonary diseases and 4 fetal lung tissues by polymerase chain reaction (PCR) and dot-blot hybridization with biotin-labelled probes. The results showed that HPV 16, 18 DNA related sequences were found in 32% of lung cancer specimens, with 10 cases of HPV 16, 5 cases of HPV 18 and 1 case of both types. 48.15% (13/27) of squamous cell carcinomas were shown to be positive for HPV 16, 18 DNA. In addition, two adenocarcinomas and one small cell carcinoma were positive for HPV 16 DNA. No specimens from benign diseases tissues and fetal lung tissues showed positive results. These results suggest that primary bronchogenic carcinoma is related to HPV infection.

摘要

在研究人乳头瘤病毒（HPV）与支气管源性癌的关系时，通过聚合酶链反应（PCR）以及用生物素标记探针进行斑点杂交，在50例肺癌患者、18例良性肺部疾病患者的样本以及4份胎儿肺组织中检测到了“高危”HPV 16、18 DNA序列。结果显示，32%的肺癌标本中发现了HPV 16、18 DNA相关序列，其中10例为HPV 16，5例为HPV 18，1例同时存在两种类型。48.15%（13/27）的鳞状细胞癌显示HPV 16、18 DNA呈阳性。此外，2例腺癌和1例小细胞癌HPV 16 DNA呈阳性。良性疾病组织和胎儿肺组织的标本均未显示阳性结果。这些结果表明原发性支气管源性癌与HPV感染有关。