Hawser S
Lepetit Research Center, Marion Merrell Dow Research Institute, Gerenzano (Varese), Italy.
J Med Vet Mycol. 1996 Mar-Apr;34(2):149-52.
Adhesion of synchronised yeast-phase Candida albicans cells to tissue culture plastic, and the susceptibility of planktonic and adherent cells to antifungal agents, was investigated using a modified tetrazolium (XTT) assay. MIC data demonstrated that ketoconazole and amphotericin B were highly active against planktonic C. albicans yeast-phase cells. XTT tetrazolium assays permitted comparisons of MIC values with XTT formazan IC50 and IC80 (percentage inhibitory concentrations); IC50 and IC80 values for amphotericin B and ketoconazole were similar. Furthermore, IC50 and IC80 values for 24 h incubation with antifungal agent were typically higher than corresponding IC50 and IC80 values for 48 h incubation. Furthermore, in comparison to values for planktonic Candida cells, adherent cells were typically less susceptible to amphotericin B and ketoconazole. For example, with increasing incubation time following the initial adhesion period, cells became progressively less susceptible to amphotericin B and ketoconazole: 24 h (P < 0.05) and 48 h (P < 0.001). Furthermore, other azoles showed the same activities compared with ketoconazole against both planktonic and adherent cells. Overall, the data demonstrate the usefulness of the XTT tetrazolium assay in describing comparisons of the susceptibility profiles for both planktonic and adherent synchronous yeast phase C. albicans in vitro.
使用改良的四氮唑（XTT）分析法，研究了同步化的白色念珠菌酵母相细胞对组织培养塑料的黏附性，以及浮游细胞和黏附细胞对抗真菌剂的敏感性。最低抑菌浓度（MIC）数据表明，酮康唑和两性霉素B对浮游的白色念珠菌酵母相细胞具有高度活性。XTT四氮唑分析法允许将MIC值与XTT甲臜IC50和IC80（抑制浓度百分比）进行比较；两性霉素B和酮康唑的IC50和IC80值相似。此外，与抗真菌剂孵育24小时的IC50和IC80值通常高于孵育48小时的相应IC50和IC80值。此外，与浮游念珠菌细胞的值相比，黏附细胞通常对两性霉素B和酮康唑的敏感性较低。例如，在初始黏附期后的孵育时间增加时，细胞对两性霉素B和酮康唑的敏感性逐渐降低：24小时（P<0.05）和48小时（P<0.001）。此外，与酮康唑相比，其他唑类对浮游细胞和黏附细胞均表现出相同的活性。总体而言，数据表明XTT四氮唑分析法在描述体外浮游和黏附同步酵母相白色念珠菌敏感性谱比较方面的有用性。
