Hawser S
Lepetit Research Centre, Gerenzano (Varese), Italy.
J Med Vet Mycol. 1996 Nov-Dec;34(6):407-10.
Adhesion of synchronized yeast-phase Candida cells to tissue culture plastic was investigated using the tetrazolium salt, XTT. The procedure permits the direct enumeration of adherent yeasts following the metabolic conversion of the XTT tetrazolium salt, to its reduced formazan form, by mitochondrial dehydrogenases. Using this procedure, the formation of XTT formazan by Candida cells was typically related to the inoculum size. The adhesion of Candida yeast-phase cells from different Candida spp. to plastic was of the following order: C. krusei (n = 5) > C. albicans (n = 10) > C. glabrata (n = 6). Furthermore, preliminary experiments with several other species indicated that C. tropicalis (n = 2) may adhere as well as C. albicans and that one strain each of C. guilliermondii and C. parapsilosis appear to adhere to plastic in a similar fashion to C. glabrata. The data indicate the utility of the XTT tetrazolium based assay in enumerating the adhesion of different Candida spp. to plastic.
利用四氮唑盐XTT研究了同步化酵母相念珠菌细胞对组织培养塑料的黏附情况。该方法可通过线粒体脱氢酶将XTT四氮唑盐代谢转化为还原型甲臜形式后，直接计数黏附的酵母细胞。采用此方法，念珠菌细胞形成XTT甲臜的情况通常与接种量有关。来自不同念珠菌属的念珠菌酵母相细胞对塑料的黏附顺序如下：克鲁斯念珠菌（n = 5）＞白色念珠菌（n = 10）＞光滑念珠菌（n = 6）。此外，对其他几个菌种的初步实验表明，热带念珠菌（n = 2）的黏附情况可能与白色念珠菌相同，季也蒙念珠菌和近平滑念珠菌各有一个菌株对塑料的黏附方式似乎与光滑念珠菌相似。数据表明基于XTT四氮唑盐的检测方法在计数不同念珠菌属对塑料的黏附方面具有实用性。
