Donzeau M, Bourdineaud J P, Lauquin G J
Institut de Biochimie et Génétique Cellularies, Centre National de la Recherche Scientifique, Bordeaux, France.
Mol Microbiol. 1996 Apr;20(2):449-59. doi: 10.1111/j.1365-2958.1996.tb02631.x.
Expression of the yeast Saccharomyces cerevisiae SRP1 (Serine-rich Protein) gene is shown here to be induced both by low temperature and anaerobic growth conditions. We show that anaerobic SRP1 expression is haem-dependent; however, haem influence does not operate through the action of the hypoxic-gene ROX1 repressor. The SRP1 promoter region displaying the stress-responsive elements is restricted to its first 551 bp, upstream of the initiation codon, although an upstream activation site contained in upstream sequences is required for full promoter activity. In addition, we demonstrate that the TIP1 gene, sharing similar nucleotide and polypeptide structure with SRP1, and previously reported to be a cold-shock-inducible gene, is also a hypoxic gene. Srp1 protein production is similarly induced by low temperature and anaerobic growth conditions. This protein, detected in the plasma membrane fraction, is shown to be exposed on the cell surface via a glycosyl-phosphatidylinositol membrane anchoring.
本文显示，酿酒酵母SRP1（富含丝氨酸蛋白）基因的表达在低温和厌氧生长条件下均会被诱导。我们发现厌氧条件下SRP1的表达依赖于血红素；然而，血红素的影响并非通过缺氧基因ROX1阻遏物的作用来实现。显示出应激反应元件的SRP1启动子区域局限于起始密码子上游的前551 bp，不过完整的启动子活性需要上游序列中包含的一个上游激活位点。此外，我们证明，TIP1基因与SRP1具有相似的核苷酸和多肽结构，且先前报道它是一个冷休克诱导基因，它也是一个缺氧基因。Srp1蛋白的产生同样在低温和厌氧生长条件下被诱导。这种在质膜组分中检测到的蛋白，经显示是通过糖基磷脂酰肌醇膜锚定暴露在细胞表面的。
