Dhar P, Jayaraman G, Karmakar N, Manchanda S C
Centre for Atmospheric Sciences, Indian Institute of Technology, New Delhi.
Med Biol Eng Comput. 1996 Mar;34(2):155-9. doi: 10.1007/BF02520021.
The effect of pressure on filtration across different de-endothelialised arteries has been studied experimentally, and the existing theoretical model is validated. Segments of different arteries are excised, de-endothelialised and cannulated. Bovine serum albumin Krebs solution is used as perfusate. Transmural water flux is measured by following the movement of an air bubble in a calibrated capillary, which connects the artery to a pressure reservoir; the pressure of which is varied. The hydraulic conductivity Lp is calculated from the flux values. Using available experimental parameters in the case of the thoracic and abdominal aorta, a theoretical model is validated using the experimental results. As the elastic constant for the carotid artery is not available, the theoretical model is used to calculate the elastic constant at different transmural pressures. The values calculated are in the range -4.9 x 10(-8) to -5.7 x 10(-9) cm2 dyne-1 between 50 and 135 mm Hg. Both theoretical and experimental results show a decrease in Lp values with an increase in transmural pressure for the thoracic and abdominal aorta, whereas a different trend is observed in the case of the carotid artery. The Lp values increase at 90 mm Hg, as compared with 50 mm Hg, and with a further increase in transmural pressure the values decrease.
已经通过实验研究了压力对不同去内皮动脉滤过的影响，并对现有的理论模型进行了验证。切除不同动脉的节段，去除内皮并插管。使用牛血清白蛋白克雷布斯溶液作为灌注液。通过跟踪校准毛细管中气泡的移动来测量跨壁水通量，该毛细管将动脉连接到压力储器，其压力是变化的。根据通量值计算水力传导率Lp。利用胸主动脉和腹主动脉情况下的可用实验参数，使用实验结果验证了理论模型。由于颈动脉的弹性常数不可用，因此使用理论模型计算不同跨壁压力下的弹性常数。计算值在50至135毫米汞柱之间，范围为-4.9×10（-8）至-5.7×10（-9）平方厘米达因-1。理论和实验结果均表明，胸主动脉和腹主动脉的Lp值随跨壁压力的增加而降低，而颈动脉则观察到不同的趋势。与50毫米汞柱相比，90毫米汞柱时Lp值增加，随着跨壁压力进一步增加，该值降低。
