Fernández R, Alba F, Ferrer J M
Department of Physiology, School of Medicine, University of Granada, Spain.
Neuroreport. 1996 Feb 29;7(3):798-800. doi: 10.1097/00001756-199602290-00028.
The purpose of this study was to examine the possible role of endogenous peptidases in the inhibition of intracranial self-stimulation (ICSS) produced by injections of neurotensin (NT) and neuromedin N (NN) into the medial prefrontal cortex (MPC) of the rat. We studied the effects on ICSS of the MPC of the administration of thiorphan and bestatin, two specific inhibitors of the peptidases that inactivate NT and NN respectively. Microinjections into MPC of thiorphan (10 micrograms) and bestatin (25 micrograms) potentiated in inhibition of ICSS produced by the intracortical administration of NT (10 nmol) and NN (20 nmol) respectively. This potentiation affected both the amplitude and the duration of the inhibition of ICSS produced by the neuropeptides. Our data indicate that endogenous peptidases are involved in the inactivation of NT and NN in the prefrontal cortex.
本研究的目的是探讨内源性肽酶在注射神经降压素（NT）和神经介素N（NN）至大鼠内侧前额叶皮质（MPC）所产生的抑制颅内自我刺激（ICSS）中可能发挥的作用。我们研究了分别使NT和NN失活的两种肽酶特异性抑制剂——硫磷酰胺和贝司他汀对MPC的ICSS的影响。向MPC微量注射硫磷酰胺（10微克）和贝司他汀（25微克）分别增强了由皮质内注射NT（10纳摩尔）和NN（20纳摩尔）所产生的对ICSS的抑制作用。这种增强作用影响了神经肽所产生的ICSS抑制的幅度和持续时间。我们的数据表明，内源性肽酶参与了前额叶皮质中NT和NN的失活过程。
