Matsunaga T, Mukhin A G, London E D
Neuroimaging and Drug Action Section, National Institute on Drug Abuse, National Institutes of Health, Baltimore, MD 21224, USA.
Neuroreport. 1996 Feb 29;7(3):833-7. doi: 10.1097/00001756-199602290-00036.
A stoichiometric analysis of N-methyl-D-aspartate (NMDA) receptor binding was conducted using [3H]dizocilpine, [3H]dichlorokynurenic acid and [3H]CGP39653, and membranes from various brain regions of rats. The ratio of the density of [3H]CGP39653 binding to [3H]dizocilpine binding was > 1 in frontal cortex and hippocampus, approximately 1 in striatum and spinal cord and < 1 in cerebellum. When [3H]dichlorokynurenic acid binding was compared with [3H]dizocilpine binding, the ratios were > 1 in frontal cortex, hippocampus and striatum, 3-4 in cerebellum, and approximately 2 in spinal cord. These observations suggest that a single channel complex may have more than one binding site for NMDA and/or glycine and that the stoichiometry between the binding domains of the NMDA receptor varies regionally.
使用[3H]地佐环平、[3H]二氯喹啉酸和[3H]CGP39653以及来自大鼠不同脑区的膜进行了N-甲基-D-天冬氨酸（NMDA）受体结合的化学计量分析。[3H]CGP39653与[3H]地佐环平结合密度的比值在额叶皮质和海马中>1，在纹状体和脊髓中约为1，在小脑中<1。当比较[3H]二氯喹啉酸与[3H]地佐环平结合时，该比值在额叶皮质、海马和纹状体中>1，在小脑中为3 - 4，在脊髓中约为2。这些观察结果表明，单个通道复合物可能对NMDA和/或甘氨酸有多个结合位点，并且NMDA受体结合域之间的化学计量在不同区域有所变化。
