Sakoff J A, Murdoch R N
Department of Biological Sciences, University of Newcastle, Callaghan N.S.W, Australia.
Biochem Mol Med. 1996 Apr;57(2):81-90. doi: 10.1006/bmme.1996.0013.
The present study was designed to test the hypothesis that Ca2+ is required for the successful induction of the decidual cell reaction (DCR) in mice following stimulation with concanavalin A (Con A). Con A (125 micrograms) administered intraluminally on Day 4 of pseudopregnancy increased uterine vascular permeability increased uterine weight, and induced morphological and histological transformations that were clearly indicative of decidualization. Radioactive CaCl2 (1 mmol liter-1, 600 mCi mmol-1 introduced into the uterine lumen with either Con A or saline was subsequently incorporated into the uterine tissue and detected only in the luminal epithelium by microautoradiography techniques. The intraluminal administration of CaCl2 in combination with Con A increased the magnitude of the lectin-induced DCR. In contrast, the administration of other cationic chloride solutions, at various concentrations and tonicity, either had no effect (viz. Na+, Mg2+, and Ba2+) or reduced (viz. K+, Zn2+, Cd2+, and La3+) this uterine response. While ionophore A23187 was also deciduogenic, it suppressed the DCR when administered before Con A and enhanced the DCR when administered after Con A. The Ca2+ channel blockers, nifedipine, verapamil, nicardipine, and diltiazem, the Ca(2+)-calmodulin inhibitor, W7, and the Ca(+)-ATPase inhibitor, thapsigargin also effectively reduced the uterine response to Con A when administered intraluminally. However, the Con A A-induced DCR was not influenced by the Ca+ chelators, EGTA, EDTA, BAPTA, and BAPTA-AM. The results confirm that Con A is deciduogenic in pseudopregnant mice and suggest that luminal Ca2+ plays an important role in facilitating the induction of the lectin-induced DCR by influencing the metabolism of the luminal epithelium.
在伴刀豆球蛋白A（Con A）刺激后，小鼠成功诱导蜕膜细胞反应（DCR）需要Ca2+。在假孕第4天经腔内给予Con A（125微克）可增加子宫血管通透性、子宫重量，并诱导形态学和组织学转变，这些转变明显表明发生了蜕膜化。放射性CaCl2（1 mmol/L，600 mCi/mmol）与Con A或生理盐水一起引入子宫腔，随后被子宫组织摄取，并仅通过微放射自显影技术在腔上皮中检测到。腔内给予CaCl2与Con A联合使用可增加凝集素诱导的DCR的幅度。相比之下，给予不同浓度和张力的其他阳离子氯化物溶液，要么没有效果（即Na+、Mg2+和Ba2+），要么降低（即K+、Zn2+、Cd2+和La3+）这种子宫反应。虽然离子载体A23187也具有诱导蜕膜的作用，但在Con A之前给药时会抑制DCR，在Con A之后给药时会增强DCR。Ca2+通道阻滞剂硝苯地平、维拉帕米、尼卡地平、地尔硫䓬，Ca(2+)-钙调蛋白抑制剂W7，以及Ca(+)-ATP酶抑制剂毒胡萝卜素经腔内给药时也能有效降低子宫对Con A的反应。然而，Con A诱导的DCR不受Ca+螯合剂乙二醇双四乙酸（EGTA）、乙二胺四乙酸（EDTA）、1,2-双（2-氨基苯氧基）乙烷-N,N,N',N'-四乙酸（BAPTA）和BAPTA-AM的影响。结果证实Con A在假孕小鼠中具有诱导蜕膜的作用，并表明腔内Ca2+通过影响腔上皮的代谢在促进凝集素诱导的DCR的诱导中起重要作用。
