Hollemann T, Schuh R, Pieler T, Stick R
Abteilung für Entwicklungsbiochemie, Universität Göttingen, Germany.
Mech Dev. 1996 Mar;55(1):19-32. doi: 10.1016/0925-4773(95)00485-8.
We have isolated an amphibian homolog of the homeotic gene spalt of Drosophila. Like its Drosophila counterpart the Xenopus Xsal-1 gene encodes a protein that contains three widely separated sets of sequence related double zinc finger motifs of the CC/HH-type as well as a single CC/HH zinc finger. The Xenopus gene encodes a fourth double zinc finger and a single CC/HC zinc finger motif that have no counterpart in the fly protein. Alternative splicing of Xsal-1 transcripts gives rise to RNAs coding for either four, three or two double zinc fingers, respectively. The main expression domains of Xsal-1 in early development are confined to distinct regions along the lateral axon tracts within the midbrain, hindbrain, and spinal cord. Outside the central nervous system Xsal-1 is expressed in the facio-acoustic ganglion and in the developing limb buds. The pattern of expression suggests that Xsal-1 might be under control of signals emanating from the notochord and/or the floor plate and that it might function in neuronal cell specification.
我们分离出了果蝇同源异型基因spalt的一种两栖动物同源物。与果蝇中的对应物一样，非洲爪蟾Xsal-1基因编码一种蛋白质，该蛋白质包含三组广泛分离的CC/HH型序列相关双锌指基序以及一个单一的CC/HH锌指。非洲爪蟾基因编码第四种双锌指和一种单一的CC/HC锌指基序，它们在果蝇蛋白质中没有对应物。Xsal-1转录本的可变剪接分别产生编码四个、三个或两个双锌指的RNA。Xsal-1在早期发育中的主要表达域局限于中脑、后脑和脊髓内沿外侧轴突束的不同区域。在中枢神经系统之外，Xsal-1在面听神经节和发育中的肢芽中表达。这种表达模式表明，Xsal-1可能受脊索和/或底板发出的信号控制，并且可能在神经元细胞特化中发挥作用。
