Boda B, Szente M
Szent-Györgyi University Medical School, Department of Neurology, Szeged, Hungary.
Neurosci Lett. 1996 May 3;209(1):37-40. doi: 10.1016/0304-3940(96)12590-8.
The effects of N-nitro-L-arginine (NA), a nitric oxide (NO) synthase inhibitor, were investigated on the focal ictal-like seizure induced by 3-aminopyridine in rat neocortex in vivo. Intraperitoneal and intracerebroventricular (i.c.v.) injections of NA markedly facilitated propagation of epileptiform events. In addition, NA injected i.c.v. increased the number/hour of individual ictal periods while decreasing their duration. In the presence of NA and an N-methyl-D-aspartate (NMDA) receptor antagonist D(-)2-amino-5-phosphonovaleric acid (APV) the number of ictal periods increased while their duration synergically decreased. APV by itself did not change the number of ictal episodes but decreased their duration. Our results suggest that NO inhibits the induction and propagation of seizure activity. We cannot distinguish the proportion of neuronal and/or vascular NO involved in our experimental conditions, but these effects seem to be independent of the NMDA receptors.
研究了一氧化氮（NO）合酶抑制剂N-硝基-L-精氨酸（NA）对大鼠体内新皮质由3-氨基吡啶诱发的局灶性癫痫样发作的影响。腹腔注射和脑室内（i.c.v.）注射NA均显著促进癫痫样事件的传播。此外，脑室内注射NA增加了单个发作期的每小时发作次数，同时缩短了其持续时间。在存在NA和N-甲基-D-天冬氨酸（NMDA）受体拮抗剂D-（-）-2-氨基-5-磷酸戊酸（APV）的情况下，发作期次数增加，而其持续时间协同缩短。单独使用APV不会改变发作次数，但会缩短其持续时间。我们的结果表明，NO抑制癫痫活动的诱导和传播。在我们的实验条件下，我们无法区分神经元性和/或血管性NO的比例，但这些作用似乎与NMDA受体无关。
