Hashimoto T, Amagai M, Murakami H, Higashiyama M, Hashimoto K, Nishikawa T
Department of Dermatology, Keio University School of Medicine, Tokyo, Japan.
Exp Dermatol. 1996 Apr;5(2):96-101. doi: 10.1111/j.1600-0625.1996.tb00101.x.
We examined 42 herpes gestationis sera with immunofluorescence of normal human skin sections, and found that anti-keratinocyte cell surface antibodies were detected specifically in 10 herpes gestationis sera. The diagnosis of these herpes gestationis cases was confirmed by detecting antibodies against the 180 kD bullous pemphigoid antigen with immunoblotting of its fusion protein. The results of immunoadsorption assay using baculoproteins of both pemphigus vulgaris and pemphigus foliaceus antigens indicated that the herpes gestationis sera did not recognize common pemphigus antigens. Immunoblotting of human epidermal extracts and immunofluorescence of various tissues also suggested that the sera did not recognize any other desmosomal components or paraneoplastic pemphigus antigens. The significance of this reactivity is unclear. However, because no control bullous pemphigoid sera showed this reactivity, it may suggest a different pathophysiology between herpes gestationis and bullous pemphigoid.
我们用正常人皮肤切片免疫荧光法检测了42份妊娠疱疹患者的血清，发现10份妊娠疱疹患者的血清中特异性检测到抗角质形成细胞表面抗体。通过对其融合蛋白进行免疫印迹检测抗180kD大疱性类天疱疮抗原的抗体，确诊了这些妊娠疱疹病例。使用寻常型天疱疮和落叶型天疱疮抗原的杆状蛋白进行免疫吸附试验的结果表明，妊娠疱疹患者的血清不识别常见的天疱疮抗原。人表皮提取物的免疫印迹和各种组织的免疫荧光也表明，这些血清不识别任何其他桥粒成分或副肿瘤性天疱疮抗原。这种反应性的意义尚不清楚。然而，由于对照大疱性类天疱疮血清未显示出这种反应性，这可能提示妊娠疱疹和大疱性类天疱疮之间存在不同的病理生理学机制。
