采用聚合酶链反应-溶液杂交酶联免疫分析法检测呼吸道标本中的卡氏肺孢子虫。
Detection of Pneumocystis carinii in respiratory specimens by PCR-solution hybridization enzyme-linked immunoassay.
作者信息
Ortona E, Margutti P, Tamburrini E, Mencarini P, Visconti E, Zolfo M, Siracusano A
机构信息
Department of Immunology, Istituto Superiore di Sanità, Rome, Italy.
出版信息
J Clin Microbiol. 1997 Jun;35(6):1589-91. doi: 10.1128/jcm.35.6.1589-1591.1997.
Abstract
By using a recently developed PCR-solution hybridization enzyme-linked assay (PCR-SHELA), we investigated Pneumocystis carinii in bronchoalveolar lavage fluid samples and induced sputa of patients with pneumocystosis. In detecting P. carinii, PCR-SHELA proved more sensitive than immunofluorescence staining or a single PCR and significantly more diagnostically specific than a nested PCR. Our data suggest that PCR-SHELA could be used to detect P. carinii organisms in respiratory samples, particularly in patients with uncertain diagnoses.
摘要
通过使用最近开发的聚合酶链反应-溶液杂交酶联测定法（PCR-SHELA），我们对肺孢子虫病患者的支气管肺泡灌洗液体样本和诱导痰液中的卡氏肺孢子虫进行了研究。在检测卡氏肺孢子虫时，PCR-SHELA比免疫荧光染色或单一聚合酶链反应更敏感，并且比巢式聚合酶链反应具有更高的诊断特异性。我们的数据表明，PCR-SHELA可用于检测呼吸道样本中的卡氏肺孢子虫病原体，特别是在诊断不明确的患者中。
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J Med Microbiol. 1996 Aug;45(2):146-8. doi: 10.1099/00222615-45-2-146.
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