Tammen I, Klippert H, Kuczka A, Treviranus A, Pohlenz J, Stöber M, Simon D, Harlizius B
Institute for Animal Breeding and Genetics, Hanover School of Veterinary Sciences, Germany.
Res Vet Sci. 1996 May;60(3):218-21. doi: 10.1016/s0034-5288(96)90042-9.
A modified DNA test, based on the polymerase chain reaction, was developed for the monogenic recessive disease bovine leucocyte adhesion deficiency (BLAD). The test was improved by the selection of new primers which facilitated the interpretation of the results. An easily scorable banding pattern makes the test useful in cattle breeding schemes and for clinical diagnosis. A total of 2381 samples was analysed over a period of three years. The carrier rate among young bulls at artificial insemination (AI) stations decreased from 11.6 per cent in 1993 to 9.9 per cent in the first five months of 1995. Continuous screening of young bulls before entering AI is still recommended unless both parents are proven to be genetically free of BLAD. The carrier rate among clinically suspect animals was not increased, and carriers are therefore not expected to be immunodeficient. Despite all efforts to eradicate the disease, calves with BLAD were still observed in 1995.
基于聚合酶链反应开发了一种改良的DNA检测方法，用于检测单基因隐性疾病牛白细胞粘附缺陷症（BLAD）。通过选择新的引物改进了该检测方法，这有助于结果的解读。易于评分的条带模式使该检测方法在牛育种计划和临床诊断中很有用。在三年时间里共分析了2381个样本。人工授精（AI）站年轻公牛的携带率从1993年的11.6%降至1995年头五个月的9.9%。除非双亲经证实基因上无BLAD，否则仍建议在年轻公牛进入人工授精之前进行持续筛查。临床疑似动物中的携带率没有增加，因此预计携带者不会免疫缺陷。尽管为根除该疾病做出了种种努力，但1995年仍观察到患有BLAD的犊牛。
