Donnay I, Devleeschouwer N, Wouters-Ballman P, Leclercq G, Verstegen J
Department of Small Animal Reproduction, Faculty of Veterinary Medicine, University of Liège, Belgium.
Res Vet Sci. 1996 May;60(3):251-4. doi: 10.1016/s0034-5288(96)90049-1.
The concentrations of receptors for epidermal growth factor (EGF-R), oestrogen (ER) and progesterone (PR) were measured in 108 samples from canine mammary tumours and 132 samples of normal mammary tissue removed surgically from 84 bitches. The history and clinical signs were also recorded. Binding sites of high affinity were detected in 70 per cent of both types of tissue and no significant variations in EGF-R concentrations or positivity were observed with the histology, location, size or number of mammary tumours or the age of the animal. A significant direct correlation (P = 0.002) was observed between the concentrations of ER and EGF-R only in malignant tumours. The concentrations of EGF-R were significantly correlated (P = 0.04) in normal mammary tissues adjacent to and distant from the lesions, but not between normal tissue and tumour tissue. No significant differences were observed in the expression of EGF-R in normal and neoplastic tissues from the same bitches. The direct correlation between the concentrations of EGF-R and ER in malignant tumours could be related to an oestrogen-dependent expression of EGF-R or to a similar pattern of regulation of the receptors.
对取自84只母犬的108份犬乳腺肿瘤样本和132份手术切除的正常乳腺组织样本，检测了表皮生长因子受体（EGF-R）、雌激素受体（ER）和孕激素受体（PR）的浓度。还记录了病史和临床症状。在两种组织类型中，均有70%检测到高亲和力结合位点，且未观察到EGF-R浓度或阳性率随乳腺肿瘤的组织学类型、位置、大小或数量以及动物年龄而有显著差异。仅在恶性肿瘤中，观察到ER和EGF-R浓度之间存在显著正相关（P = 0.002）。在病变附近和远处的正常乳腺组织中，EGF-R浓度显著相关（P = 0.04），但正常组织与肿瘤组织之间无显著相关性。在同一母犬的正常组织和肿瘤组织中，未观察到EGF-R表达有显著差异。恶性肿瘤中EGF-R和ER浓度之间的正相关可能与EGF-R的雌激素依赖性表达或受体的类似调节模式有关。
