Donnay I, Wouters-Ballman P, Devleeschouwer N, Leclercq G, Verstegen J
University of Liège, Faculty of Veterinary Medicine, Department of Small Animals Reproduction, Belgium.
Vet Res Commun. 1995;19(2):101-13. doi: 10.1007/BF01839276.
Changes in the concentrations and affinities of receptors for oestrogen (ER), progesterone (PR) and epidermal growth factor (EGF-R) were studied in mammary glands of healthy bitches with regard to age, the location in the mammary chain and the stage of the oestrous cycle. Uterus was used as the reference tissue for the evaluation of steroid receptors. Mammary and uterine samples from 7 healthy bitches were taken at five stages of the oestrous cycle in such a way that all the locations in the mammary chain were represented at each stage of the cycle (10 samples/dog). ER, PR and EGF-R were detected by biochemical assays using increasing concentrations of tritiated (steroids) or iodinated (EGF) ligands. A significant direct correlation was found between the ER and PR concentrations for mammary and uterine samples. No significant correlation was found between the steroid receptors and EGF-R concentrations. Mammary ER concentrations were significantly higher in bitches of 5 years of age or older than in younger ones; in posterior glands (4th and 5th pairs) than in anterior glands; and in the mid-luteal phase. Mammary PR did not vary significantly with age or location but was significantly lower in the early luteal phase than in other phases. A similar decrease in PR concentrations was observed in the uterus during the early luteal phase and uterine ER and PR concentrations were very low in the mid-luteal phase. Mammary EGF-R were not significantly higher in the early or mid-luteal phase than in pro-oestrus or anoestrus. The differences observed between the uterine and mammary steroid receptor concentrations during the oestrous cycle could be due to different mechanisms for regulating steroid receptor expression in the two tissues. Mammary EGF-R concentrations may be linked, as in other species, to cellular proliferation and/or to the serum progesterone concentrations.
研究了健康母犬乳腺中雌激素受体（ER）、孕激素受体（PR）和表皮生长因子受体（EGF-R）的浓度及亲和力随年龄、乳腺链位置和发情周期阶段的变化。子宫用作评估类固醇受体的参照组织。在发情周期的五个阶段采集了7只健康母犬的乳腺和子宫样本，使得乳腺链的所有位置在周期的每个阶段都有代表（每只犬10个样本）。使用浓度递增的氚标记（类固醇）或碘化（EGF）配体，通过生化测定法检测ER、PR和EGF-R。发现乳腺和子宫样本的ER与PR浓度之间存在显著的直接相关性。未发现类固醇受体与EGF-R浓度之间存在显著相关性。5岁及以上母犬的乳腺ER浓度显著高于年轻母犬；后位腺体（第4和第5对）的ER浓度高于前位腺体；且在黄体中期。乳腺PR浓度随年龄或位置无显著变化，但在黄体早期显著低于其他阶段。在黄体早期子宫中也观察到PR浓度有类似下降，且子宫ER和PR浓度在黄体中期非常低。乳腺EGF-R在黄体早期或中期并不比发情前期或乏情期显著更高。发情周期中子宫和乳腺类固醇受体浓度的差异可能是由于两种组织中调节类固醇受体表达的机制不同。乳腺EGF-R浓度可能像在其他物种中一样，与细胞增殖和/或血清孕激素浓度有关。
