Berencsi K, Gönczöl E, Endresz V, Kough J, Takeda S, Gyulay Z, Plotkin S A, Rando R F
Wistar Institute, Philadelphia, PA 19104, USA.
Vaccine. 1996 Apr;14(5):369-74. doi: 10.1016/0264-410x(95)00227-r.
We reported earlier that an adenovirus (Ad) recombinant expressing the full-length human cytomegalovirus (HCMV) glycoprotein B (gB) gene induces gB-specific cytotoxic T lymphocyte (CTL) responses in CBA (H-2k) mice (Berencsi et al., J. Gen. Virol. 74, 257-2512, 1993). Here we show that mice immunized with Ad recombinant viruses expressing truncated forms of the gB gene containing the first 700 (Ad-700), 465 (Ad-465) or 303 (Ad-303) amino acids of gB or an Ad construct containing exon 4 (E4) of the HCMV immediate early 1 (IEI) gene (Ad-IEI (E4)) demonstrate HCMV-specific CTL responses. These data suggest the importance of the first 303 amino acids of the gB polypeptide and the IEI E4 product in designing a vaccine to induce anti-HCMV CTL responses.
我们先前报道过，一种表达全长人巨细胞病毒（HCMV）糖蛋白B（gB）基因的腺病毒（Ad）重组体可在CBA（H-2k）小鼠中诱导gB特异性细胞毒性T淋巴细胞（CTL）反应（贝伦奇等人，《普通病毒学杂志》74卷，257 - 2512页，1993年）。在此我们表明，用表达gB基因截短形式的Ad重组病毒免疫的小鼠，这些截短形式包含gB的前700个（Ad - 700）、465个（Ad - 465）或303个（Ad - 303）氨基酸，或者用包含HCMV立即早期1（IE1）基因外显子4（E4）的Ad构建体（Ad - IE1（E4））免疫的小鼠，均表现出HCMV特异性CTL反应。这些数据表明，gB多肽的前303个氨基酸和IE1 E4产物在设计诱导抗HCMV CTL反应的疫苗中具有重要性。
