Borysiewicz L K, Hickling J K, Graham S, Sinclair J, Cranage M P, Smith G L, Sissons J G
Department of Medicine, Royal Postgraduate Medical School, Hammersmith Hospital, London, United Kingdom.
J Exp Med. 1988 Sep 1;168(3):919-31. doi: 10.1084/jem.168.3.919.
CTL are held to be an important host defense mechanism in persistent herpes-virus infections. We have therefore studied the nature and specificity of human cytomegalovirus (HCMV)-specific CTL in normal persistently infected individuals. This was achieved by using vaccinia recombinants encoding viral genes expressed at different stages of the virus replicative cycle, a structural glycoprotein gB (vac.gB) and the major 72-kD immediate early nonstructural protein (vac.IE) of HCMV, combined with limiting dilution analysis of the CTL response. In two subjects, 43 and 58% of HCMV CTL precursors (CTLp) lysed vac.IE-infected cells, in contrast to less than 6% lysing gB-infected cells. HCMV-specific CTL could also be generated by secondary in vitro stimulation with vac.gB- but not vac.IE-infected autologous fibroblasts. The high frequency of 72-kD IE protein-specific CTL suggests that this is at least a major recognition element for the HCMV-specific CTL response in asymptomatic persistently infected individuals, and CTL with this specificity may be important in maintaining the normal virus/host equilibrium.
细胞毒性T淋巴细胞（CTL）被认为是持续性疱疹病毒感染中重要的宿主防御机制。因此，我们研究了正常持续性感染个体中人类巨细胞病毒（HCMV）特异性CTL的性质和特异性。这是通过使用编码在病毒复制周期不同阶段表达的病毒基因的痘苗重组体来实现的，这些基因包括HCMV的一种结构糖蛋白gB（vac.gB）和主要的72-kD立即早期非结构蛋白（vac.IE），并结合CTL反应的有限稀释分析。在两名受试者中，43%和58%的HCMV CTL前体（CTLp）裂解了vac.IE感染的细胞，相比之下，裂解gB感染细胞的比例不到6%。用vac.gB而不是vac.IE感染的自体成纤维细胞进行二次体外刺激也可以产生HCMV特异性CTL。72-kD IE蛋白特异性CTL的高频率表明，这至少是无症状持续性感染个体中HCMV特异性CTL反应的主要识别元件，具有这种特异性的CTL可能在维持正常的病毒/宿主平衡中起重要作用。
