Reiss E
Infect Immun. 1977 Apr;16(1):181-8. doi: 10.1128/iai.16.1.181-188.1977.
A serial enzymatic hydrolysis procedure for the partial lysis of Histoplasma capsulatum yeast-form cell walls was described, and its application for the differentiation of two serotypes was evaluated. Cell walls were serially digested with alpha(1 leads to 3)-glucanase and beta(1 leads to 3)-glucanse of Cladosporium resinae, then by Pronase, and then by chitinase. The walls of serotype 1, 2, 3 (61.5% digested) were not susceptible to alpha(1 leads to 3)-glucanse, and they contained 30.3% chitin, thus identifying the strain as chemotype 1 (chem 1). Serotype 1, 4 cells walls (51.6% digested) released 27.3% as glucose after treatment with alpha(1 leads to 3)-glucanse and contained 7.8% chitin, compatible with chemotype 2 (chem 2). In addition to quantitating the monomeric products of enzymolysis, I recovered soluble nondialyzable polysaccharide from the digests of both serotypes.
描述了一种用于荚膜组织胞浆菌酵母型细胞壁部分裂解的连续酶促水解程序，并评估了其在两种血清型鉴别中的应用。细胞壁先用树脂枝孢菌的α(1→3)-葡聚糖酶和β(1→3)-葡聚糖酶进行连续消化，然后用链霉蛋白酶，再用几丁质酶处理。血清型1、2、3的细胞壁（消化率61.5%）对α(1→3)-葡聚糖酶不敏感，且含有30.3%的几丁质，因此将该菌株鉴定为化学型1（chem 1）。血清型1、4的细胞壁（消化率51.6%）在用α(1→3)-葡聚糖酶处理后释放出27.3%的葡萄糖，且含有7.8%的几丁质，符合化学型2（chem 2）。除了对酶解的单体产物进行定量外，我还从两种血清型的消化物中回收了可溶性不可透析多糖。
