Department of Microbiology and Bureau of Biological Research, Rutgers University, New Brunswick, New Jersey 08903.
Infect Immun. 1974 Nov;10(5):1091-7. doi: 10.1128/iai.10.5.1091-1097.1974.
Protective immunity was elicited by immunization of mice with ribosomal preparations from yeast cells of Histoplasma capsulatum. Ribosomes from disrupted cells were isolated by differential centrifugation using sodium dodecyl sulfate. These preparations contained 55% protein and 45% ribonucleic acid and sedimented as a single peak with a sedimentation coefficient of 77S on sucrose density gradient analysis. Mice immunized subcutaneously with ribosomes, with or without adjuvant, were challenged intravenously with 8 x 10(6) yeast cells of H. capsulatum. Significant protection was induced by ribosomes and was greatly enhanced by adjuvants. Protection measured by 30-day survival compared favorably with the immunoprotection assessed by absence of lung lesions and negative spleen cultures. Treatment of ribosomes with ribonuclease before immunization reduced protection by 85%, whereas trypsin and Pronase reduced the protection by 50 to 55%. These findings indicate that both intact ribosomal ribonucleic acid and protein are necessary for maximal immunogenicity of Histoplasma ribosomes.
用荚膜组织胞浆菌酵母细胞核糖体制剂免疫小鼠可诱发保护性免疫。用十二烷基硫酸钠通过差速离心从破碎细胞中分离核糖体。这些制剂含有 55%的蛋白质和 45%的核糖核酸，在蔗糖密度梯度分析中以 77S 的沉降系数沉降为单个峰。皮下用核糖体免疫，有或没有佐剂的小鼠，经静脉内用 8 x 10(6)荚膜组织胞浆菌酵母细胞攻击。核糖体诱导了显著的保护作用，佐剂大大增强了保护作用。通过 30 天的存活率来衡量的保护作用与通过无肺部病变和脾脏培养阴性来评估的免疫保护作用相当。免疫前用核糖核酸酶处理核糖体可使保护作用降低 85%，而胰蛋白酶和 Pronase 可使保护作用降低 50-55%。这些发现表明，完整的核糖体核糖核酸和蛋白质都是荚膜组织胞浆菌核糖体最大免疫原性所必需的。
