Mattioli R, Coelho J, Martins A
Departamento de Fisioterapia e Terapia Ocupacional, Universidade Federal de São Carlos, Brasil.
Braz J Med Biol Res. 1996 Apr;29(4):495-9.
The aim of the present study was to investigate whether two substance P (SP) fragments have reinforcing effects in Carassius auratus when the fish were tested in a place-preference experimental model. Fish were placed in a 3-compartment box in which one compartment gives access to two others that are not connected. The time spent in each compartment was recorded for 10 min in order to determine which one was preferred. Twenty-four hours later the fish were given one of the following ip treatments: 1) group VEH (N = 12), injected with teleost saline, 2) group DiMe-C7 (N = 12), injected with 33 micrograms/kg DiMe-C7, and 3) group SP1-7 (N = 12), injected with 167 micrograms/kg SP1-7. Immediately after treatment the fish were kept for 30 min in the compartment that was the least preferred on the day before and this procedure was repeated for 3 days. On the fifth day the fish were retested for 10 min to determine the time spent in each compartment. Two-way analysis of variance with treatments and testing as factors indicated a main effect (P < 0.0025) as well as a testing effect (P < 0.009). The post-hoc Scheffé multiple comparison test indicated that only the DiMe-C7 group presented an increase in the time spent in the paired compartment after treatment. We suggest that the C-terminal fragment of SP has reinforcing effects in Carassius auratus.
本研究的目的是在一个位置偏好实验模型中测试鱼类时，调查两种P物质（SP）片段对鲫鱼是否具有强化作用。将鱼放置在一个三室箱中，其中一个隔间可通向另外两个不相连的隔间。记录每条鱼在每个隔间停留10分钟的时间，以确定其偏好的隔间。24小时后，对鱼进行以下腹腔注射处理：1）VEH组（N = 12），注射硬骨鱼生理盐水；2）DiMe-C7组（N = 12），注射33微克/千克DiMe-C7；3）SP1-7组（N = 12），注射167微克/千克SP1-7。处理后，立即将鱼置于前一天最不喜欢的隔间中30分钟，此过程重复3天。在第五天，对鱼进行10分钟的重新测试，以确定其在每个隔间停留的时间。以处理和测试为因素的双向方差分析表明存在主效应（P < 0.0025）以及测试效应（P < 0.009）。事后Scheffé多重比较检验表明，只有DiMe-C7组在处理后在配对隔间中停留的时间增加。我们认为，SP的C末端片段对鲫鱼具有强化作用。
