Chvapil M, Stankova L, Bernhard D S, Weldy P L, Carlson E C, Campbell J B
Infect Immun. 1977 Apr;16(1):367-73. doi: 10.1128/iai.16.1.367-373.1977.
Rat peritoneal macrophages incubated in a medium with 10% autologous serum showed 50% inhibition of O2 consumption by 0.4 mM zinc only when activated by yeast. Zinc ions had no effect on resting macrophages. The inhibitory efficiency of zinc depended on the ratio of yeast particles to macrophages; with more activated cells, the inhibition was less. The multiple-shaped intact macrophages changed into rounded cells in the presence of 0.1 and 0.5 mM Zn2+ as shown by photomicrography and scanning electron microscopy. When the incubation medium was replaced by zinc-free medium, cells recovered and became irregularly shaped. The reversibility of zinc effect on cell morphology corresponded with uptake and release of zinc by macrophages. The presence of serum in the medium interfered with the magnitude of zinc uptake by macrophages. We conclude that besides already reported effects of zinc on mast cells, platelets, and granulocytes, zinc ions also inhibit some functions of macrophages.
在含有10%自体血清的培养基中培养的大鼠腹腔巨噬细胞，只有在被酵母激活时，0.4 mM锌才会使其耗氧量抑制50%。锌离子对静息巨噬细胞没有影响。锌的抑制效率取决于酵母颗粒与巨噬细胞的比例；被激活的细胞越多，抑制作用越小。光学显微镜和扫描电子显微镜显示，在存在0.1 mM和0.5 mM Zn2+的情况下，多种形态完整的巨噬细胞会变成圆形细胞。当用无锌培养基替换孵育培养基时，细胞恢复并变得形状不规则。锌对细胞形态的影响的可逆性与巨噬细胞对锌的摄取和释放相对应。培养基中血清的存在会干扰巨噬细胞对锌的摄取量。我们得出结论，除了锌对肥大细胞、血小板和粒细胞已报道的作用外，锌离子还会抑制巨噬细胞的某些功能。
