Partridge M, Kiguwa S, Luqmani Y, Langdon J D
Epithelial Cell Biology Unit, King's College School of Medicine and Dentistry, London, U.K.
Eur J Cancer B Oral Oncol. 1996 Mar;32B(2):76-82. doi: 10.1016/0964-1955(95)00056-9.
Fibroblast growth factors (FGFs) are involved in the transmission of signals between the epithelia and connective tissue, and influence epidermal growth and differentiation. They are thought to be important in the restoration of normal tissues after injury and aberrant expression may also play a role in tumorigenesis. However, no information is available on the nature of cells within oral mucosa which synthesise and/or respond to FGFs. We have screened normal oral mucosa and oral squamous cell carcinoma (SCC) for expression of bFGF by immunohistology and northern analysis and used RT-PCR to look for transcripts for KGF and the high-affinity FGF receptors FGFR1 and FGFR2. Transcripts for bFGF were detected in normal and malignant oral mucosa and KGF within connective tissue elements. The predominant FGF receptor detected in the epidermis and oral mucosa was FGFR2 which binds KGF with greater affinity than bFGF. Production of KGF by connective tissue components and synthesis of the high-affinity KGF receptor, FGFR2, by oral keratinocytes provides circumstantial evidence for a paracrine growth control loop with KGF synthesised within the lamina propria or tumour stroma influencing the proliferation and maturation of both normal oral epithelium and SCC.
成纤维细胞生长因子（FGFs）参与上皮细胞与结缔组织之间的信号传递，并影响表皮的生长和分化。它们被认为在损伤后正常组织的修复中起重要作用，异常表达也可能在肿瘤发生中发挥作用。然而，关于口腔黏膜中合成和/或对FGFs作出反应的细胞的性质尚无相关信息。我们通过免疫组织化学和Northern分析筛选了正常口腔黏膜和口腔鳞状细胞癌（SCC）中bFGF的表达，并使用逆转录聚合酶链反应（RT-PCR）寻找角质形成细胞生长因子（KGF）以及高亲和力FGF受体FGFR1和FGFR2的转录本。在正常和恶性口腔黏膜以及结缔组织成分中的KGF中检测到了bFGF的转录本。在表皮和口腔黏膜中检测到的主要FGF受体是FGFR2，它与KGF的结合亲和力高于bFGF。结缔组织成分产生KGF，口腔角质形成细胞合成高亲和力KGF受体FGFR2，这为旁分泌生长控制环提供了间接证据，即固有层或肿瘤基质中合成的KGF影响正常口腔上皮和SCC的增殖与成熟。
