Hammarström S, Troye M, Wahlund G, Svenberg T, Perlmann P
Int J Cancer. 1977 Jun 15;19(6):756-66. doi: 10.1002/ijc.2910190604.
Antibody dependent lymphocyte (K cell) mediated lysis of tumor cells in vitro was used to assay for cell surface associated carcinoembryonic antigen (CEA) and two CEA-related normal tissue components, "normal glycoprotein" (NGP) and biliary glycoprotein I (BGP I). Three tumor cell lines were used as target cells. These were HT-29, colon carcinoma, T-24, urinary bladder transitional cell carcinoma and Mel-1, malignant melanoma. To induce lysis we used the IgG-fraction of specific rabbit and monkey anti-CEA sera and of specific rabbit anti-NGP and anti-BGP I sera, respectively. Purified human lymphocytes were used as effector cells. HT-29 was efficiently killed by low concentrations of rabbit anti-CEA and less efficiently by monkey anti-CEA. T-24 and Mel-1 were not lysed by anti-CEA, HT-29 and T-24 were lysed by low concentrations of anti-BGP I. In contrast only HT-29 was lysed by anti-NGP. Only a fraction of the tumor cells was killed by the different antisera although kinetic studies showed that the lytic reaction was complete well before the end of the eighteen hour incubation period used in the assay. Anti-CEA and anti-BGP I gave 30-40% corrected lysis of HT-29. With anti-NGP the corresponding figure was 10-20%.
利用抗体依赖性淋巴细胞（K细胞）在体外介导的肿瘤细胞裂解来检测细胞表面相关的癌胚抗原（CEA）以及两种与CEA相关的正常组织成分，即“正常糖蛋白”（NGP）和胆汁糖蛋白I（BGP I）。使用了三种肿瘤细胞系作为靶细胞。它们分别是HT - 29（结肠癌）、T - 24（膀胱移行细胞癌）和Mel - 1（恶性黑色素瘤）。为诱导裂解，我们分别使用了特异性兔和猴抗CEA血清的IgG组分以及特异性兔抗NGP和抗BGP I血清的IgG组分。纯化的人淋巴细胞用作效应细胞。低浓度的兔抗CEA能有效杀死HT - 29，而猴抗CEA的杀伤效率较低。T - 24和Mel - 1不被抗CEA裂解，低浓度的抗BGP I能裂解HT - 29和T - 24。相比之下，只有HT - 29能被抗NGP裂解。尽管动力学研究表明在该检测所用的18小时孵育期结束前裂解反应就已完全，但不同抗血清仅杀死了一部分肿瘤细胞。抗CEA和抗BGP I对HT - 29的校正裂解率为30 - 40%。抗NGP的相应数值为10 - 20%。
