Sewerynek E, Ortiz G G, Reiter R J, Pablos M I, Melchiorri D, Daniels W M
Department of Cellular and Structural Biology, University of Texas Health Science Center, San Antonio 78284-7762, USA.
Mol Cell Endocrinol. 1996 Mar 25;117(2):183-8. doi: 10.1016/0303-7207(95)03742-x.
The ability of melatonin to influence lipopolysaccharide (LPS)-induced genotoxicity was tested using micronuclei as an index in both bone marrow and peripheral blood cells of rats. LPS was given as a single dose of 10 mg/kg. Melatonin (5 mg/kg) was injected prior to LPS administration and thereafter at 6 h intervals to the conclusion of the study (72 h). The number of micronucleated polychromatic erythrocytes increased significantly after LPS administration both in cells from peripheral blood and bone marrow. Melatonin administration to LPS-treated rats highly significantly reduced micronuclei formation in both peripheral blood and bone marrow cells beginning at 24 h after LPS administration and continuing to the end of the study. In blood the increase in micronuclei formation was time-dependent in LPS-treated rats with peak values being reached at 36-48 h. The ability of melatonin to reduce LPS-related genotoxicity is likely related to its antioxidant activity.
以微核为指标，在大鼠骨髓细胞和外周血细胞中测试了褪黑素影响脂多糖（LPS）诱导的遗传毒性的能力。LPS以10 mg/kg的单剂量给药。在给予LPS之前注射褪黑素（5 mg/kg），之后每隔6小时注射一次，直至研究结束（72小时）。给予LPS后，外周血和骨髓细胞中的微核多染红细胞数量显著增加。对接受LPS处理的大鼠给予褪黑素，从给予LPS后24小时开始，直至研究结束，均能极显著地减少外周血和骨髓细胞中的微核形成。在血液中，LPS处理的大鼠微核形成的增加呈时间依赖性，在36 - 48小时达到峰值。褪黑素降低LPS相关遗传毒性的能力可能与其抗氧化活性有关。
