Zhang Y G, Liu T P
Department of Pharmacology, Nanjing Medical University, China.
Zhongguo Yao Li Xue Bao. 1996 Jan;17(1):44-8.
To study the influences of ginsenosides Rb1 and Rg1 (active components of the total saponins of Panax ginseng) on the brains against ischemia-reperfusion injury.
Rat focal cerebral ischemia was induced by reversible middle cerebral artery occlusion (MCAO) without craniectomy. The influences of ginsenoside Rb1 and Rg1 on infarct size (IS), neurologic deficit (ND) and the contents of calcium and potassium in the infarct were observed.
In a 2-h ischemia, Rb1 10-40 mg.kg-1 i.v. 30 min before MCAO decreased IS by 20%-49% and ND score from 5.1 to 4.1-2.3, and inhibited Ca accumulation and K loss by 22%-50% and 18-37%, respectively; Rb1 10-40 mg.kg-1 i.v. immediately after MCA was recanalized decreased IS by 12%-35% and ND score from 5.2 to 4.3-3.3, and inhibited Ca accumulation and K loss by 10%-40% and 17%-30%, respectively. In permanent ischemia, Rb1 40 mg.kg-1 i.v. reduced IS, ND, Ca accumulation and K loss. However, Rg1 40 mg.kg-1 i.v. did not show effect on both permanent and 2-h MCAO.
Rb1 protected brain from ischemic and reperfusion injuries.
研究人参皂苷Rb1和Rg1（人参总皂苷的活性成分）对脑缺血再灌注损伤的影响。
采用可逆性大脑中动脉闭塞（MCAO）法制备大鼠局灶性脑缺血模型，不进行开颅手术。观察人参皂苷Rb1和Rg1对梗死体积（IS）、神经功能缺损（ND）以及梗死灶内钙和钾含量的影响。
在2小时缺血模型中，MCAO前30分钟静脉注射10 - 40mg·kg-1的Rb1可使IS降低20% - 49%，ND评分从5.1降至4.1 - 2.3，并分别抑制钙蓄积和钾丢失22% - 50%和18% - 37%；MCA再通后立即静脉注射10 - 40mg·kg-1的Rb1可使IS降低12% - 35%，ND评分从5.2降至4.3 - 3.3，并分别抑制钙蓄积和钾丢失10% - 40%和17% - 30%。在永久性缺血模型中，静脉注射40mg·kg-1的Rb1可减少IS、ND、钙蓄积和钾丢失。然而，静脉注射40mg·kg-1的Rg1对永久性和2小时MCAO均无作用。
Rb1可保护脑免受缺血和再灌注损伤。
