依他酸和钙霉素对培养的人肺成纤维细胞中DNA和胶原蛋白合成的影响。
Effects of egtazic acid and calcimycin on synthesis of DNA and collagen in cultured human lung fibroblasts.
作者信息
Liu H L, Lu H M, Li D G, Qi F, Jiang Z M
机构信息
Department of Internal Medicine, Xin-Hua Hospital, Shanghai Second Medical University, China.
出版信息
Zhongguo Yao Li Xue Bao. 1996 Jan;17(1):63-5.
AIM
To study the effects of egtazic acid (EA) and calcimycin (Cal) on the synthesis of DNA and collagen in cultured human lung fibroblasts (HLF).
METHODS
The synthesis of DNA and collagen was determined by measuring the incorporation of [3H]TdR and [3H]proline of HLF respectively.
RESULTS
The collagen synthesis increased markedly 24 h after exposure to both EA (0.05-4 mmol.L-1) and Cal (0.25-20 mumol.L-1), and that there was no obvious change in DNA synthesis. After 36-48-h exposure, both DNA and collagen syntheses decreased in the groups of EA (1, 2, and 4 mmol.L-1); the DNA synthesis was also suppressed in Cal groups in a concentration-dependent manner, whereas collagen synthesis decreased only in Cal (10 and 20 mumol.L-1).
CONCLUSION
Extracellular Ca2+ influx into fibroblasts increased collagen production, However, the DNA synthesis was suppressed when the cytosolic Ca2+ was too high or too low.
目的
研究依他酸（EA）和A23187（Cal）对培养的人肺成纤维细胞（HLF）中DNA和胶原蛋白合成的影响。
方法
分别通过测量HLF中[3H]TdR和[3H]脯氨酸的掺入量来测定DNA和胶原蛋白的合成。
结果
暴露于EA（0.05 - 4 mmol·L-1）和Cal（0.25 - 20 μmol·L-1）24小时后，胶原蛋白合成显著增加，而DNA合成无明显变化。暴露36 - 48小时后，EA（1、2和4 mmol·L-1）组的DNA和胶原蛋白合成均下降；Cal组的DNA合成也呈浓度依赖性受到抑制，而胶原蛋白合成仅在Cal（10和20 μmol·L-1）组下降。
结论
细胞外Ca2+流入成纤维细胞增加了胶原蛋白的产生，然而，当胞质Ca2+过高或过低时，DNA合成受到抑制。
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