Kokaia Z, Nawa H, Uchino H, Elmér E, Kokaia M, Carnahan J, Smith M L, Siesjö B K, Lindvall O
Department of Neurology, University Hospital, Lund, Sweden.
Brain Res Mol Brain Res. 1996 May;38(1):139-44. doi: 10.1016/0169-328x(96)00002-2.
Levels of BDNF mRNA and protein were measured in the rat brain using in situ hybridization and a two-site enzyme immunoassay. Under basal conditions, the highest BDNF concentration was found in the dentate gyrus (88 ng/g), while the levels in CA3 (50 ng/g), CA1 (18 ng/g) and parietal cortex (8 ng/g) were markedly lower. Following 10 min of forebrain ischemia, BDNF protein increased transiently in the dentate gyrus (to 124% of control at 6 h after the insult) and CA3 region (to 131% of control, at 1 week after the insult). In CA1 and parietal cortex, BDNF protein decreased to 73-75% of control at 24 h. In contrast, BDNF mRNA expression in dentate granule cells and CA3 pyramidal layer was transiently elevated to 287 and 293% of control, respectively, at 2 h, whereas no change was detected in CA1 or neocortex. The regional BDNF protein levels shown here correlate at least partly with regional differences in cellular resistance to ischemic damage, which is consistent with the hypothesis of a neuroprotective role of BDNF.
采用原位杂交和双位点酶免疫分析法测定大鼠脑内BDNF mRNA和蛋白水平。在基础条件下，齿状回中BDNF浓度最高（88 ng/g），而CA3（50 ng/g）、CA1（18 ng/g）和顶叶皮质（8 ng/g）中的水平则明显较低。在前脑缺血10分钟后，齿状回中BDNF蛋白短暂增加（损伤后6小时达到对照的124%），CA3区也增加（损伤后1周达到对照的131%）。在CA1和顶叶皮质中，BDNF蛋白在24小时时降至对照的73 - 75%。相反，齿状颗粒细胞和CA3锥体细胞层中的BDNF mRNA表达在2小时时分别短暂升高至对照的287%和293%，而在CA1或新皮质中未检测到变化。此处所示的区域BDNF蛋白水平至少部分与细胞对缺血损伤的抗性区域差异相关，这与BDNF具有神经保护作用的假设一致。
