Garcia-Suarez O, Naves F J, Del Valle M E, Esteban I, Bronzetti E, Vazquez E, Vega J A
Departamento de Morfología y Biología Celular, Facultad de Medicina, Universidad de Oviedo, Spain.
Anat Embryol (Berl). 1996 Jun;193(6):577-83. doi: 10.1007/BF00187929.
We investigated the expression of immunoreactivity (IR) for low- (p75) and high-affinity (trk proteins) neurotrophin-receptor proteins in adult human paravertebral-sympathetic ganglion neurons. Mouse monoclonal antibodies against the pan-neurotrophin-receptor p75, and rabbit polyclonal antibodies against specific epitopes of the intracytoplasmic domain on trk neurotrophin-receptor proteins were used in fresh unfixed and formaldehyde-fixed paraffin-embedded sympathetic ganglia. All adult human paravertebral-sympathetic neurons displayed trkA neurotrophin-receptor-like protein IR, 10% express trkC neurotrophin-receptor-like protein IR, 37-44% show p75 IR, and no IR was obtained for trkB neurotrophin-receptor-like protein. The intensity of immunostaining was independent of the neuron size. Labelling of non-neuronal tissues, especially blood-vessel walls, was observed for p75, trkA and trkC neurotrophin-receptor proteins. These results indicate that overlapping exists in the expression of p75 and trk neurotrophin-receptor proteins in adult human paravertebral-sympathetic neurons, and suggest that neurotrophins might act on these neurons.
我们研究了成人椎旁交感神经节神经元中低亲和力（p75）和高亲和力（trk蛋白）神经营养因子受体蛋白的免疫反应性（IR）表达。针对泛神经营养因子受体p75的小鼠单克隆抗体，以及针对trk神经营养因子受体蛋白胞质内结构域特定表位的兔多克隆抗体，被用于新鲜未固定和甲醛固定的石蜡包埋交感神经节。所有成人椎旁交感神经元均显示trkA神经营养因子受体样蛋白IR，10%表达trkC神经营养因子受体样蛋白IR，37 - 44%显示p75 IR，而trkB神经营养因子受体样蛋白未获得IR。免疫染色强度与神经元大小无关。观察到p75、trkA和trkC神经营养因子受体蛋白对非神经元组织，尤其是血管壁有标记。这些结果表明成人椎旁交感神经元中p75和trk神经营养因子受体蛋白的表达存在重叠，并提示神经营养因子可能作用于这些神经元。
