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用于表征膳食气味化合物向血浆和乳汁转移的气相色谱-质谱法。

Gas chromatographic-mass spectrometric method to characterise the transfer of dietary odorous compounds into plasma and milk.

作者信息

Désage M, Schaal B, Soubeyrand J, Orgeur P, Brazier J L

机构信息

Laboratoire d'Etudes Analytiques et Cinétiques du Médicament, Université Claude Bernard, Lyon, France.

出版信息

J Chromatogr B Biomed Appl. 1996 Apr 12;678(2):205-10. doi: 10.1016/0378-4347(95)00527-7.

DOI:10.1016/0378-4347(95)00527-7
PMID:8738023
Abstract

The flavours contained in a mammalian mother's milk can exert a marked influence on her offspring's proximate suckling behaviour and later preferences. The aim of this study was to establish a reliable analytical procedure to characterise the mammary transfer of selected volatile constituents of maternal food from non-pregnant and recently parturient ewes. Six known volatile compounds, most representative of cumin aroma (alpha-pinene, gamma-terpinene, cuminaldehyde, p-cymene, limonene and cineole), were traced in the blood and milk of ewes fed with cumin seeds, using liquid-liquid extraction combined with gas chromatography-specific ion monitoring mass spectrometry. Among the six cumin odour markers, only one, p-cymene, was transferred in quantifiable amounts into the venous plasma. The other cumin markers could only be detected as traces corresponding to amounts lower that the limit of quantification. In milk, four of the cumin markers could be detected, and two of these were quantified.

摘要

哺乳动物母乳中的风味物质可对其后代近期的吮乳行为及日后的偏好产生显著影响。本研究的目的是建立一种可靠的分析方法,以表征未怀孕和刚分娩的母羊将母体食物中选定挥发性成分进行乳腺转运的情况。使用液液萃取结合气相色谱-特定离子监测质谱法,对喂食孜然种子的母羊的血液和乳汁中的六种已知挥发性化合物进行了追踪,这六种化合物最能代表孜然香气(α-蒎烯、γ-松油烯、枯茗醛、对伞花烃、柠檬烯和桉叶素)。在这六种孜然气味标志物中,只有对伞花烃以可量化的量转移至静脉血浆中。其他孜然标志物只能检测到痕量,其含量低于定量限。在乳汁中,可以检测到四种孜然标志物,其中两种得到了定量。

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