兔出血症病毒（RHDV）：肝脏匀浆中典型颗粒和类核心颗粒的超微结构及生化研究

Rabbit hemorrhagic disease virus (RHDV): ultrastructure and biochemical studies of typical and core-like particles present in liver homogenates.

作者信息

Granzow H, Weiland F, Strebelow H G, Liu C M, Schirrmeier H

机构信息

Federal Research Centre for Virus Diseases of Animals, Friedrich-Loeffler-Institutes, Insel Riems, Germany.

出版信息

Virus Res. 1996 Apr;41(2):163-72. doi: 10.1016/0168-1702(96)01285-3.

DOI:10.1016/0168-1702(96)01285-3

PMID:8738175

Abstract

Calicivirus particles isolated from rabbits suffering from acute RHD were compared with virions found in rabbits with chronic disease. Liver homogenates of rabbits with the protracted disease display no hemagglutinating activity and contain viral particles with diameters of 25-27 nm. These virions contain only one structural protein of 30 kDa and are distinctly smaller than intact rabbit hemorrhagic disease virus (RHDV) (32-40 nm). To prove the RHDV identity of the smaller virions, their reactivity with RHDV specific antibodies was investigated by immunoblots of the virion protein and by immunoelectron microscopy. Proteolytic digestion of RHDV particles with alpha-chymotrypsin did not transform RHDV into the smaller form. We assume that these core-like particles (CLPs) are not a result of proteolytic digestion but arise from a truncated RHDV genome or defective expression.

摘要

将从患有急性兔病毒性出血症（RHD）的兔子中分离出的杯状病毒颗粒与患有慢性疾病的兔子体内发现的病毒粒子进行了比较。患有迁延性疾病的兔子的肝脏匀浆没有血凝活性，并且含有直径为25 - 27纳米的病毒颗粒。这些病毒粒子仅包含一种30千道尔顿的结构蛋白，并且明显小于完整的兔出血症病毒（RHDV）（32 - 40纳米）。为了证明较小病毒粒子的RHDV身份，通过病毒粒子蛋白的免疫印迹和免疫电子显微镜研究了它们与RHDV特异性抗体的反应性。用α-胰凝乳蛋白酶对RHDV颗粒进行蛋白水解消化并没有将RHDV转化为较小的形式。我们假设这些核心样颗粒（CLPs）不是蛋白水解消化的结果，而是来自截短的RHDV基因组或缺陷表达。