Schnieder T, Epe C, von Samson-Himmelstjerna G
Institute of Parasitology, School of Veterinary Medicine, Hannover, Germany.
Parasitol Res. 1996;82(5):392-4. doi: 10.1007/s004360050134.
The bovine lungworm Dictyocaulus viviparus is found in cattle and deer species (roe deer, fallow deer). Although a separate lungworm species (D. eckerti) from roe deer has been described, this description, based on minor morphological differences, is not generally accepted. The aim of the present study was to examine genetic differences among four lungworm species isolated from cattle, sheep, horses, and fallow deer. Ribosomal second internal transcribed spacers (ITS2) of individual worms of all species were amplified by polymerase chain reaction (PCR), and restriction-fragment-length polymorphisms were analyzed after digestion with five different restriction enzymes. After electrophoretic separation of the digested PCR products, all four lungworm species showed different banding patterns. Lungworms from cattle (D. viviparus) and those from fallow deer (D. eckerti) were genetically distinct. These results are consistent with the hypothesis that the taxonomic classification of D. eckerti as a separate species is correct.
牛肺线虫（Dictyocaulus viviparus）存在于牛和鹿类物种（狍、黇鹿）中。尽管已描述了一种来自狍的独立肺线虫物种（D. eckerti），但基于微小形态差异的这一描述并未被普遍接受。本研究的目的是检测从牛、羊、马和黇鹿中分离出的四种肺线虫物种之间的遗传差异。通过聚合酶链反应（PCR）扩增所有物种单个虫体的核糖体第二内部转录间隔区（ITS2），并用五种不同的限制酶消化后分析限制性片段长度多态性。对消化后的PCR产物进行电泳分离后，所有四种肺线虫物种均显示出不同的条带模式。来自牛的肺线虫（D. viviparus）和来自黇鹿的肺线虫（D. eckerti）在基因上是不同的。这些结果与将D. eckerti分类为一个独立物种的假设是正确的这一观点一致。
