Włodarski K H, Kuzaka B, Włodarski P
Department of Histology and Embryology, Institute of Biostructure, Medical School, Warsaw, Poland.
World J Urol. 1996;14 Suppl 1:S16-20. doi: 10.1007/BF00182059.
Small pieces (ca. 2-3 x 3-5 mm) of the urinary-tract mucosa from noninvasive papillary transitional-cell carcinomas of the bladder (ca. urotheliale papillare, n = 33), invasive transitional-cell carcinomas of the bladder (ca. urotheliale papillare infiltrans, n = 6, papillary transitional-cell carcinomas of the bladder with squamous metaplasia (ca. urotheliale papillare cum metaplasia planoepitheliale, n = 4), transitional-cell carcinomas in situ (ca. urotheliale in situ, n = 2), and squamous-cell carcinomas of the bladder (ca. planoepitheliale, n = 2) were grafted intramuscularly into cortisone-immunosuppressed mice to test the ability of transformed transitional epithelium to induce heterotopic osteogenesis. Altogether, 156 implants from 47 cases of urinary bladder carcinoma were performed. Histological examination of implants, excised 10-17 days later, revealed relatively good survival of the grafted epithelium, which had proliferated and, in some cases, formed cysts and islands but failed to induce heterotopic osteogenesis in the surrounding host tissues. In nine implants prepared from four cases (noninvasive papillary transitional-cell carcinoma of the bladder and invasive papillary transitional-cell carcinoma of the bladder, two cases each) a small amount of cartilage and/or bone was found in the stroma of grafted tissue. The rarity of this phenomenon--together with the observation that implants of normal human urinary-tract mucosa have never induced the formation of cartilage/bone, whereas in a similar system, dog or guinea-pig grafts are osteogenic--suggests that the cartilage/bone present in the stroma of implanted cancers is the result of metaplasia of the stroma of the neoplasm and not the product of any osteoinductive potency of human urothelium.
取膀胱非侵袭性乳头状移行细胞癌（约尿路上皮乳头状瘤，n = 33）、膀胱侵袭性移行细胞癌（约尿路上皮乳头状浸润癌，n = 6）、伴有鳞状化生的膀胱乳头状移行细胞癌（约尿路上皮乳头状瘤伴鳞状化生，n = 4）、原位移行细胞癌（约尿路上皮原位癌，n = 2）以及膀胱鳞状细胞癌（约鳞状上皮癌，n = 2）的小块（约2 - 3×3 - 5毫米）尿路黏膜，肌肉内移植到经皮质酮免疫抑制的小鼠体内，以测试转化的移行上皮诱导异位骨生成的能力。总共对47例膀胱癌进行了156次植入。10 - 17天后切除植入物进行组织学检查，结果显示移植的上皮细胞存活相对良好，上皮细胞已增殖，在某些情况下形成囊肿和岛状结构，但未能在周围宿主组织中诱导异位骨生成。在由4例（膀胱非侵袭性乳头状移行细胞癌和膀胱侵袭性乳头状移行细胞癌各2例）制备的9个植入物中，在移植组织的基质中发现了少量软骨和/或骨。这种现象的罕见性——以及正常人类尿路黏膜植入物从未诱导软骨/骨形成的观察结果，而在类似系统中，狗或豚鼠的移植具有成骨性——表明植入癌基质中存在的软骨/骨是肿瘤基质化生的结果，而非人类尿路上皮任何骨诱导潜能的产物。
