Sastre J, Olmo M, Novalvos A, Ibañez D, Lahoz C
Servicios de Alergia e Inmunologia, Fundacion Jimenez Diaz, Madrid, Spain.
Allergy. 1996 Feb;51(2):117-20. doi: 10.1111/j.1398-9995.1996.tb04568.x.
We describe a 27-year-old subject who developed rhinitis and asthma symptoms 1 year after starting to prepare a certain kind of sausage. He was previously diagnosed as having allergy to coconut, banana, and kiwi and allergic rhinitis to horse, cat, dog, and cow. A positive immediate skin prick test (SPT) for paprika (dry powder of Capsicum annuum [Solanaceae]), coriander (Coriandrum sativum [umbelliferous]), and mace (shell of nutmeg, Myristica fragrans [Myristicaceae]) at a concentration of 10% (w/v) was obtained. SPT with other sausage ingredients, mites, pollens, and molds were negative. By ELISA, specific IgE antibodies to paprika, coriander, and mace were demonstrated. By ELISA-inhibition assays, a partial cross-reactivity was found among IgE-binding components from paprika and mace. The immunoblot analysis showed two IgE-reactive protein bands able to bind to IgE from mace of 20 and 40 kDa and two other bands from coriander extract of 50 and 56 kDa. No bands were detected from paprika extract. Specific bronchial inhalation challenges showed an immediate asthmatic reaction to extracts from paprika, coriander, and mace with a maximum fall in FEV1 of 26%, 40%, and 31%, respectively, with no late asthmatic reactions. In summary, we demonstrate that inhalation of dust from paprika, coriander, and mace can result in an IgE-mediated reaction to these spices. In this patient, occupational asthma was due to spices from botanically unrelated species.
我们描述了一名27岁的受试者，他在开始制作某种香肠1年后出现了鼻炎和哮喘症状。他之前被诊断对椰子、香蕉和猕猴桃过敏，对马、猫、狗和牛过敏且患有过敏性鼻炎。对辣椒粉（辣椒 [茄科] 的干粉）、芫荽（芫荽 [伞形科]）和肉豆蔻衣（肉豆蔻的外壳，肉豆蔻 [肉豆蔻科]）进行浓度为10%（w/v）的即时皮肤点刺试验（SPT）呈阳性。对其他香肠成分、螨虫、花粉和霉菌进行的SPT为阴性。通过酶联免疫吸附测定（ELISA），证实了对辣椒粉、芫荽和肉豆蔻衣的特异性IgE抗体。通过ELISA抑制试验，发现辣椒粉和肉豆蔻衣的IgE结合成分之间存在部分交叉反应。免疫印迹分析显示，肉豆蔻衣中有两条能与20 kDa和40 kDa的IgE结合的IgE反应性蛋白条带，芫荽提取物中有另外两条50 kDa和56 kDa的条带。辣椒粉提取物中未检测到条带。特异性支气管吸入激发试验显示，对辣椒粉、芫荽和肉豆蔻衣提取物有即时哮喘反应，FEV1分别最大下降26%、40%和31%，无迟发性哮喘反应。总之，我们证明吸入辣椒粉、芫荽和肉豆蔻衣的粉尘可导致对这些香料的IgE介导反应。在该患者中，职业性哮喘是由植物学上不相关物种的香料引起的。
