玉米粉尘所致职业性哮喘中IgE结合成分的鉴定
Identification of IgE-binding components in occupational asthma caused by corn dust.
作者信息
Park H S, Nahm D H
机构信息
Department of Allergy and Clinical Immunology, Ajou University School of Medicine, Suwon, Korea.
出版信息
Ann Allergy Asthma Immunol. 1997 Jul;79(1):75-9. doi: 10.1016/S1081-1206(10)63089-X.
BACKGROUND
There have been some reports of corn dust-induced occupational asthma that suggest nonimmunologic mechanism. In this paper, we present a case of occupational asthma and rhinitis caused by corn dust where bronchoconstriction was induced by an IgE-mediated reaction.
METHODS AND RESULTS
The patient had positive responses to weed and grass pollens as well as corn dust extracts. The bronchoprovocation test elicited an early asthmatic response to corn dust extracts. Serum-specific IgE and IgG4 antibodies to corn dust extracts were detected by enzyme-linked immunosorbent assay (ELISA). In order to identify further the allergenic component of the extracts, sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and electroblotting studies were performed. Ten IgE-binding components (9 to 140 kD) were detected within the corn dust extracts.
CONCLUSION
These results suggest that inhalation of corn dust can cause IgE-mediated bronchoconstriction in an exposed worker.
背景
已有一些关于玉米粉尘诱发职业性哮喘的报道，提示存在非免疫机制。在本文中，我们报告一例由玉米粉尘引起的职业性哮喘和鼻炎病例，其中支气管收缩是由IgE介导的反应诱发的。
方法与结果
该患者对杂草和草花粉以及玉米粉尘提取物呈阳性反应。支气管激发试验引发了对玉米粉尘提取物的早期哮喘反应。通过酶联免疫吸附测定（ELISA）检测到血清中针对玉米粉尘提取物的特异性IgE和IgG4抗体。为了进一步鉴定提取物的致敏成分，进行了十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）和电印迹研究。在玉米粉尘提取物中检测到10种IgE结合成分（9至140kD）。
结论
这些结果表明，吸入玉米粉尘可导致接触该粉尘的工人发生IgE介导的支气管收缩。
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