抗体作为研究牛血清白蛋白重折叠的免疫探针。各结构域内的重折叠。
Antibody as immunological probe for studying refolding of bovine serum albumin. Refolding within each domain.
作者信息
Teale J M, Benjamin D C
出版信息
J Biol Chem. 1977 Jul 10;252(13):4521-6.
PMID:873903
Abstract
Antiserum to bovine serum albumin was fractionated into populations of antibody directed against party, an apparent pathway of refolding within each domain was obtained which is consistent with the proposed evolutionary pathway of the albumin molecule. The COOH-terminal one-third of each domain refolds faster than the NH2-terminal two-thirds of each respective domain. In addition, further evidence in given for a correlation between the rate of refolding and the degree of interdomain influence that might restrict refolding.
摘要
将抗牛血清白蛋白抗血清分离成针对部分的抗体群体，获得了每个结构域内明显的重折叠途径，这与白蛋白分子提出的进化途径一致。每个结构域的羧基末端三分之一比重折叠各自结构域氨基末端的三分之二快。此外，还给出了进一步的证据，证明重折叠速率与可能限制重折叠的结构域间影响程度之间存在相关性。
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