Nieto F S, Watkins W B, Lopata A, Baker H W, Edgar D H
Reproductive Biology Unit, Royal Women's Hospital, Carlton, Victoria, Australia.
J Assist Reprod Genet. 1996 May;13(5):386-9. doi: 10.1007/BF02066169.
The aim of this study was to examine the influence of endometrial cells on the fertilization rate and early embryonic morphology following routine in vitro fertilization (IVF). Cryopreservation with subsequent thawing allowed the use of autologous somatic cells, thus minimizing the risk of transmission of infective agents. Interpatient variability was eliminated by randomizing oocytes from each cycle into the control or coculture group.
Two hundred ninety-four oocytes from 24 IVF cycles (21 patients) were included in the study (145 coculture and 149 control). The normal fertilization rate of control oocytes (56.4%) was not significantly different from that of oocytes cocultured with endometrial cells (61.4%). The mean number of blastomeres in cocultured embryos (3.65) was not significantly different from the number in control embryos (3.46) 2 days after insemination, but the proportion of embryos with minimal or no fragmentation was significantly higher in the coculture group [34/84 (40.5%) vs. 17/80 (21.3%); P < 0.01].
The inclusion of cryopreserved autologous endometrial cells in routine clinical IVF procedures does not influence fertilization or the early cleavage rate but may reduce the extent of embryo fragmentation during the early cleavage divisions.
本研究旨在探讨在常规体外受精（IVF）后，子宫内膜细胞对受精率和早期胚胎形态的影响。通过冷冻保存随后解冻，可以使用自体体细胞，从而将感染因子传播的风险降至最低。通过将每个周期的卵母细胞随机分配到对照组或共培养组，消除了患者间的变异性。
本研究纳入了来自24个IVF周期（21例患者）的294个卵母细胞（145个共培养组和149个对照组）。对照组卵母细胞的正常受精率（56.4%）与与子宫内膜细胞共培养的卵母细胞的受精率（61.4%）无显著差异。受精后2天，共培养胚胎中的卵裂球平均数量（3.65）与对照胚胎中的卵裂球平均数量（3.46）无显著差异，但共培养组中碎片极少或无碎片的胚胎比例显著更高[34/84（40.5%）对17/80（21.3%）；P<0.01]。
在常规临床IVF程序中加入冷冻保存的自体子宫内膜细胞不会影响受精或早期卵裂率，但可能会降低早期卵裂期胚胎的碎片程度。
