Gook D A, Osborn S M, Bourne H, Johnston W I
Reproductive Biology Unit, Royal Women's Hospital, Carlton, Victoria, Australia.
Hum Reprod. 1994 Apr;9(4):684-91. doi: 10.1093/oxfordjournals.humrep.a138572.
Survival following cryopreservation of fresh and aged human oocytes by the propanediol (PROH) procedure was observed in 51 and 73% of oocytes respectively, immediately after thawing. This survival was reduced in both types of oocytes at the time of insemination (3-4 h) to 41% in fresh and 61% in aged oocytes. Insemination of the cryopreserved and control oocytes with spermatozoa from one donor resulted in total fertilization rates similar to our in-vitro fertilization (IVF) rate for non-male factor patients. The normal fertilization rate for fresh cryopreserved oocytes was slightly lower (46%) than the rate for IVF oocytes (59%) (P < 0.05), while the abnormal fertilization rates were not significantly different (16 and 15% respectively). In contrast, a reduction in the normal fertilization rate was observed for the aged cryopreserved oocytes (13%) compared to the IVF rate (P < 0.001). Associated with this was an increase in the abnormal fertilization rate for the aged cryopreserved oocytes, which was significantly higher (47%) than the IVF rate (15%) (P < 0.001). Significant differences in the total and normal fertilization rates were observed between cryopreserved oocytes obtained from cohorts with < or = 27 (total: 84%, normal: 68%) and > 27 oocytes (total: 55%, normal: 33%) (P < 0.05). Fertilized oocytes and oocytes with abnormal or absent spindles were examined for chromosomal loss and no stray chromosomes were observed in any of these cryopreserved oocytes (n = 137). In the cryopreserved oocytes which had undergone normal fertilization, four scorable karyotypes were achieved and in all of these two sets of 23 chromosomes were observed.
通过丙二醇（PROH）程序对新鲜和老化的人类卵母细胞进行冷冻保存后，解冻后立即观察到的存活率分别为51%和73%。在授精时（3 - 4小时），这两种类型的卵母细胞的存活率均有所下降，新鲜卵母细胞降至41%，老化卵母细胞降至61%。用来自一名供体的精子对冷冻保存的卵母细胞和对照卵母细胞进行授精，其总受精率与我们对非男性因素患者的体外受精（IVF）率相似。新鲜冷冻保存的卵母细胞的正常受精率略低于IVF卵母细胞（46%对59%）（P < 0.05），而异常受精率无显著差异（分别为16%和15%）。相比之下，与IVF率相比，老化冷冻保存的卵母细胞的正常受精率有所降低（13%）（P < 0.001）。与此相关的是，老化冷冻保存的卵母细胞的异常受精率增加，显著高于IVF率（47%对15%）（P < 0.001）。从卵母细胞数量≤27个（总计：84%，正常：68%）和>27个卵母细胞的队列中获得的冷冻保存卵母细胞之间，总受精率和正常受精率存在显著差异（总计：55%，正常：33%）（P < 0.05）。对受精的卵母细胞以及纺锤体异常或缺失的卵母细胞进行染色体丢失检查，在任何这些冷冻保存的卵母细胞（n = 137）中均未观察到游离染色体。在经历正常受精的冷冻保存卵母细胞中，获得了四种可评分的核型，并且在所有这些核型中均观察到两组23条染色体。
