Tseng J, Li T L
Department of Biology, National Taiwan Normal University, Taipei, Taiwan.
Am J Chin Med. 1996;24(1):45-52. doi: 10.1142/S0192415X96000074.
Si-Jun-Zi-Tang is one of the widely used Chinese herbal medicines. In this study, human peripheral blood monocytes were treated in vitro with 50% hot ethanol extract of Si-Jun-Zi-Tang and its four major ingredients (Dangshen, Baizhu, Gancao and Fuling). The concentration of granulocyte-macrophage colony-stimulating factor (GM-CSF) in the culture supernatant at 3 hours and 18 hours were measured using an ELISA. Dangshen and Gancao significantly suppressed GM-CSF secretion in a dose-dependent manner. Baizhu showed no statistically significant effect on GM-CSF secretion 18 hours after in vitro drug-treatment. Fuling, by contrast, significantly augmented GM-CSF secretion in a dose-dependent manner after 18 hours of drug treatment. Si-Jun-Zi-Tang showed a suppressive effect on GM-CSF secretion at 3 hours but significantly augmented GM-CSF secretion when the cells were treated with 8 mg/ml of the drug for 18 hours. The data suggested that Si-Jun-Zi-Tang might modulate hematopoiesis and immune response via regulating GM-CSF secretion, and the presence of Fuling in Si-Jun-Zi-Tang could counteract the suppressive effect of Dangshen and Gancao on GM-CSF secretion.
四君子汤是一种广泛使用的中药。在本研究中，用人外周血单核细胞在体外分别用四君子汤及其四种主要成分（党参、白术、甘草和茯苓）的50%热乙醇提取物进行处理。使用酶联免疫吸附测定法（ELISA）测量培养上清液中3小时和18小时时粒细胞-巨噬细胞集落刺激因子（GM-CSF）的浓度。党参和甘草以剂量依赖的方式显著抑制GM-CSF的分泌。白术在体外药物处理18小时后对GM-CSF的分泌没有统计学上的显著影响。相比之下，茯苓在药物处理18小时后以剂量依赖的方式显著增加GM-CSF的分泌。四君子汤在3小时时对GM-CSF的分泌有抑制作用，但当细胞用8mg/ml的药物处理18小时时，显著增加GM-CSF的分泌。数据表明，四君子汤可能通过调节GM-CSF的分泌来调节造血和免疫反应，且四君子汤中茯苓的存在可抵消党参和甘草对GM-CSF分泌的抑制作用。
