加味四君子汤通过增加巨噬细胞来抑制结肠癌肝转移。
The modified Si-Jun-Zi Decoction attenuates colon cancer liver metastasis by increasing macrophage cells.
机构信息
The Affiliated Hospital of Nanjing University of Chinese Medicine, Jiangsu Province Hospital of Chinese Medicine, Nanjing, 210029, Jiangsu, China.
Nanjing University of Chinese Medicine, Nanjing, 210023, Jiangsu, China.
出版信息
BMC Complement Altern Med. 2019 Apr 23;19(1):86. doi: 10.1186/s12906-019-2498-4.
BACKGROUND
The modified Si-Jun-Zi Decoction (SJZ), a Chinese medicine formula, is clinically used against multiple malignancies including colorectal cancer (CRC). This study aims to evaluate the effect of modified SJZ on CRC liver metastasis and identify the therapeutic mechanisms.
METHODS
Human CRC cells with GFP fluorescence were transplanted into Balb/c nude mice spleens. Modified SJZ, 5-fluorouracil or the combined treatment was given for 3 weeks. CRC liver metastasis was measured by fluorescence imaging and plasma cytokines were analyzed. Furthermore, the effects of administration time and doses for the modified SJZ were investigated in nude mice.
RESULTS
Modified SJZ could increase the survival rate and reduce CRC liver metastasis in the nude mice model. Plasma GM-CSF level was elevated. Three weeks of treatment with the modified SJZ at the full dose (45 g/kg) could significantly increase the number of macrophages but not neutrophils in the spleen.
CONCLUSIONS
These results indicate that modified SJZ can inhibit CRC liver metastasis by activating the innate immune system, providing a complementary and alternative therapy for CRC.
背景
改良四君子汤（SJZ）是一种中药方剂，临床上用于治疗多种恶性肿瘤，包括结直肠癌（CRC）。本研究旨在评估改良 SJZ 对 CRC 肝转移的作用，并探讨其治疗机制。
方法
将带有 GFP 荧光的人 CRC 细胞移植到 Balb/c 裸鼠脾脏中。给予改良 SJZ、5-氟尿嘧啶或联合治疗 3 周。通过荧光成像测量 CRC 肝转移，分析血浆细胞因子。进一步在裸鼠中研究改良 SJZ 的给药时间和剂量的影响。
结果
改良 SJZ 可提高裸鼠模型的存活率并降低 CRC 肝转移。血浆 GM-CSF 水平升高。全剂量（45g/kg）治疗 3 周可显著增加脾脏中巨噬细胞而非中性粒细胞的数量。
结论
这些结果表明，改良 SJZ 通过激活先天免疫系统抑制 CRC 肝转移，为 CRC 提供了一种补充和替代治疗方法。
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